在微型高密度皮科升反应器中进行基因组测序
Marcel Margulies1, Michael Egholm, William E Altman
1454 Life Sciences Corp., 20 Commercial Street, Branford, Connecticut 06405, USA.
Nature
|August 2, 2005
概括
一个新的DNA测序系统比桑格测序的吞吐量增加了100倍. 这种可扩展的并行系统可以实现高精度的快速基因组测序,从而减少时间和成本.
科学领域:
- 基因组学就是基因组学.
- 分子生物学分子生物学
- 生物技术是生物技术.
背景情况:
- 大规模的DNA测序项目需要更快,更具成本效益的方法.
- 目前的测序技术,如毛细管电泳,在吞吐量和成本方面面临限制.
研究的目的:
- 开发一个可扩展,高度并行的DNA测序系统.
- 显著增加原始吞吐量,减少测序时间和成本.
主要方法:
- 使用了一种新的光纤幻灯片,带有单个井,用于并行处理.
- 开发了一种基于乳液的DNA放大方法.
- 实施了一种针对固体支和picolitre体积进行合成测序的优化热测序协议.
主要成果:
- 实现的原始吞吐量明显高于最先进的毛细管电泳仪器.
- 在四小时的运行中,测序了2500万个基数,准确率为99%或更高.
- 通过枪测序和Mycoplasma genitalium基因组的de novo组装证明了实用性,吞吐量,准确性和稳健性,一次运行96%的覆盖率达到99.96%的准确性.
结论:
- 开发的系统在DNA测序吞吐量和效率方面取得了实质性的进步.
- 这项技术为大规模的基因组学项目提供了强大而准确的解决方案.
- 该系统有可能显著减少与DNA测序相关的时间和成本.
相关概念视频
03:00Next-generation Sequencing
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.
03:21RNA-seq
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...