灵长类C型病毒的核酸序列在正常和瘤的人类组织中
1Department of Pathology, University of Chicago, Illinois 60637, USA.
Nature
|May 12, 1977
概括
人类胚胎肺纤维细胞释放一种C型病毒,HEL-12,与灵长类病毒免疫相关. 有证据表明,这种病毒不是污染物,但可能与人类癌症有关.
科学领域:
- 病毒学 病毒学
- 在瘤学瘤学.
- 免疫学 免疫学 免疫学
背景情况:
- 从人类细胞中分离出来的C型病毒因与灵长类C型病毒的相似性引起争议.
- 以前在人体细胞中发现的C型病毒往往被视为污染,尽管反复隔离.
研究的目的:
- 为了证明从人类胚胎肺纤维细胞中分离出来的HEL-12型C病毒不是偶然的污染物.
- 调查HEL-12病毒与人类疾病 (包括白血病和系统性红斑狼) 之间的潜在联系.
主要方法:
- 在HEL-12病毒抗原和人体组织之间进行免疫交叉反应性测定.
- 在骨髓性白血病患者中检测针对逆转录酶的特定抑制性免疫球蛋白.
- 在系统性红斑狼患者中对HEL-12相关抗原的球性免疫复合物的分析.
- 分子杂交技术用于检测HEL-12细胞中的前病毒DNA序列和癌症患者DNA中的同源RNA序列.
主要成果:
- 骨髓性白血病患者的循环免疫球蛋白抑制了HEL-12和灵长类型C病毒逆转录酶.
- 来自系统性红斑狼患者的淋巴细胞免疫复合体含有与HEL-12相关的抗原.
- 在可检测的抗原表达或病毒释放之前,在HEL-12细胞中检测到Proviral DNA序列.
- 在某些癌症患者的DNA中发现了与HEL-12病毒RNA相同的核酸序列.
结论:
- HEL-12病毒很可能是一种内源性人类逆转录病毒,而不是污染物.
- 在人类组织和癌症中存在HEL-12相关抗原和核酸序列,这表明它可能在人类疾病中发挥作用.
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