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相关概念视频

Polarity of the Cytoskeleton01:18

Polarity of the Cytoskeleton

The intrinsic polarity of cells can be primarily attributed to two factors- i) the asymmetric accumulation of mobile components such are regulatory molecules and subcellular components across the cell and ii) the orientation of polar cytoskeletal filaments that make up the cytoskeletal networks, specifically microfilaments, and microtubules arranged along the axis of polarity. Interactions between the cytoskeletal filaments are crucial for the establishment and maintenance of the polar nature...
Centrosome Duplication02:25

Centrosome Duplication

The primary microtubule organizing center (MTOC) in animal cells is the centrosome. A centrosome has two cylindrical centrioles at its core. Each centriole consists of nine sets of three microtubules held together by proteins. The centrioles are positioned at right angles to each other and surrounded by a shapeless protein cloud called the pericentriolar matrix, or pericentriolar material (PCM).
To ensure that each daughter cell receives a centrosome after cell division, centrosome duplication...
Determining the Plane of Cell Division02:13

Determining the Plane of Cell Division

Positioning the cell division plane is a critical step during development and cell differentiation, particularly during mitosis when the plane is essential for determining the size of the two daughter cells. The cell division plane is perpendicular to the plane of chromosome segregation, but different types of organisms have different cell division mechanisms to suit their morphology and function. 
Animal cells
In animal cells, the cleavage furrow forms along the plane of cell division starting...
Cell Polarization by Rho Proteins01:21

Cell Polarization by Rho Proteins

Cell polarity is the asymmetric distribution of cellular and membrane components, making one side of the cell different from the other. This polarity is essential to many processes such as embryogenesis, axon migration, glucose transport across epithelial cells, and directional cell migration. A migrating cell responds to intracellular or extracellular signals via molecular cascades that reorganize the actin cytoskeleton to establish this polarity. In these cells, the Rho family proteins Cdc42,...
Centrosome Duplication02:25

Centrosome Duplication

The primary microtubule organizing center (MTOC) in animal cells is the centrosome. A centrosome has two cylindrical centrioles at its core. Each centriole consists of nine sets of three microtubules held together by proteins. The centrioles are positioned at right angles to each other and surrounded by a shapeless protein cloud called the pericentriolar matrix, or pericentriolar material (PCM).
To ensure that each daughter cell receives a centrosome after cell division, centrosome duplication...
Centrioles and Centrosomes01:13

Centrioles and Centrosomes

Most animal cells comprise a pair of centrioles together called a centrosome. The cell duplicates its centrosome and contains two centrosomes side-by-side, which begin to move apart during the prophase. As the centrosomes migrate to two different sides of the cell, microtubules start extending from each centrosome toward the other end. The mitotic spindle is composed of the centrosomes and their emerging microtubules.
Near the end of the prophase, also called late prophase or "prometaphase,"...

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相关实验视频

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Live Imaging of Drosophila Larval Neuroblasts
09:50

Live Imaging of Drosophila Larval Neuroblasts

Published on: July 7, 2014

中心细胞的定位决定了神经元的极性.

Froylan Calderon de Anda1, Giulia Pollarolo, Jorge Santos Da Silva

  • 1Cavalieri Ottolenghi Scientific Institute, Universita degli Studi di Torino, 10043 Orbassano, Torino, Italy.

Nature
|August 5, 2005
PubMed
概括

神经元两极分化是由线粒分裂后第一个形成的神经元所指导的. 不对称的器官动态,特别是涉及中心体,对于建立这种关键的神经元极性至关重要.

科学领域:

  • 神经科学是一个神经科学.
  • 细胞生物学 细胞生物学
  • 发展生物学 发展生物学

背景情况:

  • 神经元的两极分化,特别是轴突的形成,是细胞分裂后的关键过程.
  • 决定轴突特异性的机制仍然不清楚,关于内在与外在线索的争论仍在进行中.
  • 了解神经元极性是理解大脑发育和功能的关键.

研究的目的:

  • 研究 hippocampal 神经元中控制神经元偏振的内在机制.
  • 确定有机体定位和动态在轴突规范中的作用.
  • 为了阐明神经元两极分化是否依赖于预先编程的细胞事件.

主要方法:

  • 在实验室中分化海马神经元的实时成像.
  • 分离后的有机细胞分布 (中心体,高尔基体,内体体) 的分析.
  • 功能性试验涉及操纵中心细胞数量和功能.

主要成果:

  • 轴突始终由线粒分裂后最早发育的神经元形成.
  • 中心体,戈尔吉体和内体体聚集在线粒体平面对面,在神经元生长之前.
  • 极化微管聚合和膜运输发生在神经元形成之前.
  • 中心细胞数量的增加导致多个轴突的形成.

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In Situ Visualization of Axon Growth and Growth Cone Dynamics in Acute Ex Vivo Embryonic Brain Slice Cultures
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In Situ Visualization of Axon Growth and Growth Cone Dynamics in Acute Ex Vivo Embryonic Brain Slice Cultures

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Live-Cell Imaging of Drosophila melanogaster Third Instar Larval Brains

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Live Imaging of Drosophila Larval Neuroblasts
09:50

Live Imaging of Drosophila Larval Neuroblasts

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In Situ Visualization of Axon Growth and Growth Cone Dynamics in Acute Ex Vivo Embryonic Brain Slice Cultures
10:45

In Situ Visualization of Axon Growth and Growth Cone Dynamics in Acute Ex Vivo Embryonic Brain Slice Cultures

Published on: October 14, 2021

Live-Cell Imaging of Drosophila melanogaster Third Instar Larval Brains
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Live-Cell Imaging of Drosophila melanogaster Third Instar Larval Brains

Published on: June 23, 2023

  • 抑制中枢细胞介导功能可以防止神经元的两极分化.
  • 结论:

    • 在早期的转基因后阶段,非对称的,以中心体为媒介的器官活力学指导神经元极性.
    • 这种内在机制表明细胞分裂方向和随后的神经元两极化之间存在联系.
    • 这些发现强调了亚细胞组织在神经元发育中的重要性.