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精子表面β-1,4-银酸转移酶和卵膜ZP3之间的互补性介导了精子-卵结合
D J Miller1, M B Macek, B D Shur
1Department of Biochemistry and Molecular Biology, University of Texas M.D. Anderson Cancer Center, Holcombe, Houston 77030.
Nature
|June 18, 1992
概括
鼠标精子β-1,4-银酸转移酶 (Gal-转移酶) 与ZP3结合,调节受精. 这种相互作用对于精子与卵子的结合至关重要,并且在受精后的青体反应或ZP3修饰后丢失.
科学领域:
- 生殖生物学 生殖生物学
- 分子生物学分子生物学
- 葡萄糖生物学 葡萄糖生物学
背景情况:
- 哺乳动物雌激体识别的分子机制尚未完全理解.
- β-1,4-银酸转移酶 (Gal-转移酶) 存在于细胞表面,并与特定的糖化物基质结合.
- 之前的研究表明,精子上的Gal-转移酶通过ZP3糖蛋白结合卵子外 (透明区).
研究的目的:
- 确认精子Gal转移酶和ZP3是否是互补的配体受体,调解精子与卵子的结合.
- 调查Gal-转移酶-ZP3相互作用在小鼠受精中的特异性和作用.
主要方法:
- 研究了精子Gal转移酶与 zona pellucida 糖蛋白的结合特异性.
- 研究了阻断或去除ZP3.3上的Gal转移酶结合部位的影响.
- 检查了转移酶局部化后的青体反应和ZP3修饰后的受精.
主要成果:
- 鼠标精子Gal转移酶特别识别具有精子结合活性的ZP3寡糖.
- 非精子的Gal-转移酶表现出更广泛的结合到 zona pellucida 糖蛋白,这表明精子酶的特异性更高.
- 阻止ZP3上的Gal-转移酶结合部位抑制了精子结合.
- 体反应引起了Gal转移酶的重新定位,阻止了ZP3的结合.
- 在受精后的ZP3修改消除了Gal转移酶结合部位,减少了精子受体活性.
结论:
- 精子表面β-1,4-银酸转移酶和ZP3是互补的粘附分子,在小鼠中介于初级伴生体结合.
- 这种相互作用对于初始的精子-卵子识别和受精期间的结合至关重要.
- 转移酶局部化和ZP3结构的变化在整个受精过程中调节性质组结合.
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