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Assaying Protein Kinase Activity with Radiolabeled ATP
Published on: May 26, 2017
在激活细胞外信号调节激酶2的p21ras的参与
A M de Vries-Smits1, B M Burgering, S J Leevers
1Laboratory for Physiological Chemistry, University of Utrecht, The Netherlands.
Nature
|June 18, 1992
概括
拉斯原瘤基因产物 (Ras) 激活细胞外信号调节激酶 (ERKs) 对生长因子信号传递至关重要. 拉斯调解胰岛素和血小板衍生生长因子 (PDGF) 诱导的ERK激活.
科学领域:
- 细胞信号传递途径 细胞信号传递途径
- 分子生物学分子生物学
- 瘤基因研究研究
背景情况:
- 增长因子激活细胞外信号调节激酶 (ERKs),也称为MAP激酶.
- 增长因素也激活了p21ras (Ras),通过促进其从GDP-bound转换到GTP-bound形式.
- 直接引入p21ras上蛋白迅速激活ERKs,这表明它在生长因子信号传递中的作用.
研究的目的:
- 研究p21ras在调解生长因子诱导的ERK激活中的作用.
- 确定Ras是否是不同生长因子信号通路的共同调解者.
主要方法:
- 使用了一种表达p21ras (RasAsn17) 干扰突变的重组疫苗病毒.
- 评估了RasAsn17对NIH3T3细胞中胰岛素诱导的ERK2和酸-3-酶激活的作用.
- 研究了RasAsn17对由血小板衍生生长因子 (PDGF) 和鼠1细胞中的醇诱导的ERK2活性的影响.
主要成果:
- RasAsn17完全抑制了NIH3T3细胞中的胰岛素诱导的ERK2激活,这些细胞过度表达胰岛素受体.
- RasAsn17没有抑制胰岛素诱导的酸-3-酶激活.
- RasAsn17抑制了大鼠-1细胞中PDGF诱导的ERK2活性,但没有醇诱导的ERK2活性.
结论:
- p21ras (Ras) 是胰岛素和PDGF的信号通路中的关键调解器.
- 拉斯通过这些生长因子专门调解ERK2的激活.
- 这些发现突出了Ras在将特定生长因子受体与ERK激活联系的核心作用.
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