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相关概念视频

Ligand Binding and Linkage00:49

Ligand Binding and Linkage

Allosteric proteins have more than one ligand binding site; the binding of a ligand to any of these sites influences the binding of ligands to the other sites. When a protein is allosteric, its binding sites are called coupled or linked.  In the case of enzymes, the site that binds to the substrate is known as the active site and the other site is known as the regulatory site. When a ligand binds to the regulatory site, this leads to conformational changes in the protein that can influence the...
Mechanical Protein Function01:58

Mechanical Protein Function

Proteins perform many mechanical functions in a cell. These proteins can be classified into two general categories- proteins that generate mechanical forces and proteins that are subjected to mechanical forces. Proteins providing mechanical support to the structure of the cell, such as keratin, are subjected to mechanical force, whereas proteins involved in cell movement and transport of molecules across cell membranes, such as an ion pump, are examples of generating mechanical force. 
Structural Protein Function01:56

Structural Protein Function

Structural proteins are a category of proteins responsible for functions ranging from cell shape and movement to providing support to major structures such as bones, cartilage, hair, and muscles. This group includes proteins such as collagen, actin, myosin, and keratin.
Collagen, the most abundant protein in mammals, is found throughout the body. In connective tissue, such as skin, ligaments, and tendons, it provides tensile strength and elasticity.  In bones and teeth, it mineralizes to form...
Role of Matrix Metalloproteases in Degradation of ECM01:23

Role of Matrix Metalloproteases in Degradation of ECM

Matrix metalloproteases (MMPs) are enzymes involved in the hydrolysis of proteins and glycoproteins of the extracellular matrix. MMPs are essential for the migration and proliferation of cells through the dense matrix network, throughout embryonic development, and throughout morphogenesis. The first MMP activity discovered was a collagenase in a tadpole's tail undergoing metamorphosis. The active collagen deposition and modifications lead to the morphogenesis of tadpoles into the adult body.
A...
Laminins are the Adhesive Proteins of Basal Lamina00:55

Laminins are the Adhesive Proteins of Basal Lamina

Laminins are heterotrimeric proteins with high molecular mass found in the extracellular matrix. Each laminin molecule is composed of three chains, viz. alpha, beta, and gamma, coded by five, four, and three paralogous genes, respectively. Laminins are categories based on the compositions of the three chains.
In humans, the five forms of alpha chains are LAMA 1, LAMA 2, LAMA 3, LAMA 4, and LAMA 5. The four forms of beta chains are LAMB 1, LAMB 2, LAMB 3, and LAMB 4. The three forms of gamma...
Transducer Mechanism: Enzyme-Linked Receptors01:27

Transducer Mechanism: Enzyme-Linked Receptors

Enzyme-linked receptors are cell-surface receptors acting as an enzyme or associating with an enzyme intracellularly. They make excellent drug targets. Drugs can bind to the extracellular ligand-binding domain or directly affect their enzymatic domain and alter their activity.
Major types that are helpful drug targets include:

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相关实验视频

Updated: Jul 8, 2026

Metabolic Labeling of Leucine Rich Repeat Kinases 1 and 2 with Radioactive Phosphate
11:31

Metabolic Labeling of Leucine Rich Repeat Kinases 1 and 2 with Radioactive Phosphate

Published on: September 18, 2013

尼卡斯特林作为一个玛分泌酶基质受体的功能.

Sanjiv Shah1, Sheu-Fen Lee, Katsuhiko Tabuchi

  • 1Center for Basic Neuroscience, The University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA.

Cell
|August 13, 2005
PubMed
概括

尼卡斯特林作为粉样蛋白前体蛋白 (APP) 和诺奇基质的受体起作用. 它的ectodomain结合了裂开的基质,将它们招募到玛分泌酶复合体中,用于膜内蛋白质溶解.

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Specificity Analysis of Protein Lysine Methyltransferases Using SPOT Peptide Arrays
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Nanosensors to Detect Protease Activity In Vivo for Noninvasive Diagnostics
10:50

Nanosensors to Detect Protease Activity In Vivo for Noninvasive Diagnostics

Published on: July 16, 2018

相关实验视频

Last Updated: Jul 8, 2026

Metabolic Labeling of Leucine Rich Repeat Kinases 1 and 2 with Radioactive Phosphate
11:31

Metabolic Labeling of Leucine Rich Repeat Kinases 1 and 2 with Radioactive Phosphate

Published on: September 18, 2013

Specificity Analysis of Protein Lysine Methyltransferases Using SPOT Peptide Arrays
08:48

Specificity Analysis of Protein Lysine Methyltransferases Using SPOT Peptide Arrays

Published on: November 29, 2014

Nanosensors to Detect Protease Activity In Vivo for Noninvasive Diagnostics
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科学领域:

  • 分子和细胞生物学分子和细胞生物学
  • 神经科学是一个神经科学.

背景情况:

  • 玛分泌酶对粉样蛋白前体蛋白 (APP) 和Notch.进行膜内裂解.
  • 尼卡斯特林是已知的玛分泌酶复合物的组成部分,其作用尚不确定.

研究的目的:

  • 阐明尼卡斯特林在玛分泌酶复合体中的功能.
  • 调查基质如何被招募用于玛分泌酶介导的裂变.

主要方法:

  • 研究了尼卡斯特林ectodomain和基质氨基末端之间的相互作用.
  • 使用化学和抗体阻断策略.
  • 进行了尼卡斯特林ectodomain的突变分析.

主要成果:

  • 尼卡斯特林的ectodomain绑定了新生成的氨基末端的切割APP和Notch基板.
  • 这种结合有利于将基质招募到玛分泌酶复合体中.
  • 阻止这种相互作用或突变尼卡斯特林ectodomain会减少基质结合和裂变.

结论:

  • 尼卡斯特林作为一种受体,在ectodomain脱落后作用于I型跨膜蛋白的氨基终端的受体.
  • 尼卡斯特林将这些基质呈现给玛分泌酶,促进它们的内膜蛋白解.