相关实验视频

Updated: Jul 26, 2026

Lipid Vesicle-mediated Affinity Chromatography using Magnetic Activated Cell Sorting (LIMACS): a Novel Method to Analyze Protein-lipid Interaction
07:33

Lipid Vesicle-mediated Affinity Chromatography using Magnetic Activated Cell Sorting (LIMACS): a Novel Method to Analyze Protein-lipid Interaction

Published on: April 26, 2011

珠结合蛋白质体:用于对膜蛋白-连接体相互作用的nmr分析的复制方法

Mariko Yokogawa1, Koh Takeuchi, Ichio Shimada

  • 1Graduate School of Pharmaceutical Sciences, The University of Tokyo, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.

Journal of the American Chemical Society
|August 25, 2005
PubMed
概括

这项研究引入了一种用于研究膜蛋白相互作用的新方法,使用亲和珠上的脂质双层,保留原生蛋白质结构和功能,用于NMR分析. 这种技术成功地绘制了KcsA通道和激毒素2.2之间的相互作用.

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Native Cell Membrane Nanoparticles System for Membrane Protein-Protein Interaction Analysis07:31

Native Cell Membrane Nanoparticles System for Membrane Protein-Protein Interaction Analysis

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相关概念视频

¹H NMR: Complex Splitting01:13

¹H NMR: Complex Splitting

A proton M that is coupled to a proton X results in doublet signals for M. However, NMR-active nuclei can be simultaneously coupled to more than one nonequivalent nucleus. When M is coupled to a second proton A, such as in styrene oxide, each peak in the doublet is split into another doublet.
Splitting diagrams or splitting tree diagrams are routinely used to depict such complex couplings. While drawing splitting diagrams, the splitting with the larger coupling constant is usually applied first.