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相关概念视频

UV–Vis Spectroscopy of Conjugated Systems01:32

UV–Vis Spectroscopy of Conjugated Systems

Organic compounds with conjugated double bonds show strong absorption features in the UV–visible region of the electromagnetic spectrum attributed to π → π* electronic excitations. Generally, a UV–vis absorption spectrum is recorded as a plot of absorbance vs wavelength. The wavelength of maximum absorbance, which manifests as a peak in the absorption spectrum, is denoted as λmax.
One of the factors influencing λmax is the extent of conjugation in the...
UV–Vis Spectrometers01:14

UV–Vis Spectrometers

The absorbance of UV and visible (UV–visible) radiations is measured using a UV–visible spectrophotometer. Deuterium lamps, which emit UV radiation, and tungsten lamps, which produce radiation in the visible region, are used as light sources in UV–visible spectrophotometers. A monochromator or prism is used for diffraction grating, i.e., to split the incoming radiation into different wavelengths. A system of slits is used to focus the desired wavelength on the sample cell. Samples for...

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在真空中, schiff-base视网膜染色体的吸收.

Lars H Andersen1, Iben B Nielsen, Michael B Kristensen

  • 1Department of Physics and Astronomy, University of Aarhus, DK-8000 Aarhus C, Denmark. lha@phys.au.dk

Journal of the American Chemical Society
|September 1, 2005
PubMed
概括

质子化视网膜的吸收光谱在真空中测量,显示最大在610nm. 这一发现挑战了以前与基于溶剂的测量方法进行的比较,并澄清了视网膜蛋白质的光谱调整.

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科学领域:

  • 生物物理学的生物物理.
  • 摄影化学的使用.
  • 分子光谱学 分子光谱学

背景情况:

  • 视网膜蛋白质对于视觉和能量传导至关重要,表现出不同的光谱特性.
  • 以前的研究将视网膜染色体光谱与甲醇 (440 nm) 中的测量进行了比较,这种方法现在被证明是误导性的.
  • 了解罗多普辛的光谱调整是阐明它们功能的关键.

研究的目的:

  • 在真空中准确测量其生物相关的质子化希夫基形式的全跨视网膜染色体的吸收光谱.
  • 建立一个可靠的基准频谱用于光谱调节在 rhodopsins,免费的环境影响.
  • 重新评估在视网膜蛋白质中调色背后的分子机制.

主要方法:

  • 全透视视网膜染色体在其质子化希夫基形式的气相吸收光谱.
  • 测量了带有中性希夫基的视网膜模型染色体的吸收光谱.
  • 气相数据与以前基于溶剂的测量数据的比较.

主要成果:

  • 质子化希夫基视网膜染色体的最大吸收在真空中被确定为610nm.
  • 气相数据提供了无电荷和双极的参考值,与甲醇的440nm最大值形成对比.
  • 罗多普辛中的蛋白质环境被证明是蓝色转移,而不是红色转移,吸收光谱.

结论:

  • 气相吸收数据为了解视网膜蛋白质的光谱调节提供了更准确的参考.
  • 罗多普辛中的蛋白质环境诱导了吸收光谱的蓝色转移.
  • 这些发现解释了在metharhodopsin I和bacteriorhodopsin中观察到的光谱变化.