Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

The Early Endosome: Endocytosis of Transferrin01:28

The Early Endosome: Endocytosis of Transferrin

Essential proteins such as insulin or low-density lipoprotein (LDL) and micronutrients such as iron enter a eukaryotic cell through receptor-mediated endocytosis. Subsequently, the early endosomes fuse with the vesicles containing such receptor-ligand complexes and play a vital role in sorting the incoming ligands and receptors. While the ligands are either degraded inside the vesicle or released into the cytosol, their receptors are returned to the plasma membrane for further rounds of...
Recycling Endosomes and Transcytosis00:58

Recycling Endosomes and Transcytosis

The recycling endosome, also known as the endosomal recycling compartment (ERC), is a part of the slow-recycling process of the endocytic pathway. Molecules internalized through receptor-mediated endocytosis are either degraded in the lysosomes or are recycled to the plasma membrane through the fast- or slow-recycling route.
The recycling endosome is not a single organelle but an extensively tubulated network of recycling pathways. It functions in storing molecules or transporting them across...
Rab Proteins01:14

Rab Proteins

Rab proteins constitute the largest family of monomeric GTPases, of which 70 members are present in humans. Rab proteins and their effectors regulate consecutive stages of vesicle transport such as vesicle transport, docking, and fusion to the correct recipient membrane.
Rab proteins switch between a cytosolic, GDP-bound inactive state and a membrane-anchored, GTP-bound active state. By themselves, Rabs show slow rates of GDP/GTP exchange and GTP hydrolysis. Thus, Rab proteins are considered...
Cell Polarization by Rho Proteins01:21

Cell Polarization by Rho Proteins

Cell polarity is the asymmetric distribution of cellular and membrane components, making one side of the cell different from the other. This polarity is essential to many processes such as embryogenesis, axon migration, glucose transport across epithelial cells, and directional cell migration. A migrating cell responds to intracellular or extracellular signals via molecular cascades that reorganize the actin cytoskeleton to establish this polarity. In these cells, the Rho family proteins Cdc42,...
Rab Cascades01:25

Rab Cascades

Rab GTPases act in a regulated cascade during membrane fusion, helping the lipid bilayers mix. The Rab family of proteins are active when bound to GTP, and inactive when bound to GDP. Hence, they act as guanine nucleotide-dependent molecular switches. Rab-GTP recognizes and binds to long or short-range tethering proteins to capture the target vesicle. These tethers coordinate with SNAREs on the vesicle and the target membrane to assemble the trans SNARE complex that locks the mixing bilayers.

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Sometimes a Long Way to Go: Symptomatic Subtle Nonosseous Capitotrapezoid Coalition. Case Report and Literature Review.

Seminars in musculoskeletal radiology·2026
Same author

Tobacco smoke but not e-cigarette vapor induces epithelial barrier disruption, inflammation, and DNA damage in human Calu-3 cells.

Scientific reports·2026
Same author

A supracellular actin network transmits forces over long distances at the apical surface of squamous carcinoma cells.

Journal of cell science·2026
Same author

Common and rare variant analyses reveal genetic factors underlying idiopathic pulmonary fibrosis and its shared aetiology with severe COVID-19.

EBioMedicine·2025
Same author

AICAr Inhibition of cardiomyocyte autophagy promotes p62-dependent NRF2 expression and protection against doxorubicin toxicity.

Cellular and molecular life sciences : CMLS·2025
Same author

Adipose Triglyceride Lipase Knockout Increases Anticontractile Effects of Perivascular Adipose Tissue.

Arteriosclerosis, thrombosis, and vascular biology·2025

相关实验视频

Updated: Jul 1, 2026

Live-cell Imaging of Sensory Organ Precursor Cells in Intact Drosophila Pupae
04:27

Live-cell Imaging of Sensory Organ Precursor Cells in Intact Drosophila Pupae

Published on: May 27, 2011

不对称的Rab 11内基因组调节三角形循环,并确定Drosophila神经系统中的细胞命运.

Gregory Emery1, Andrea Hutterer, Daniela Berdnik

  • 1Institute of Molecular Biotechnology of the Austrian Academy of Sciences, Dr. Bohr Gasse 3-5, 1030 Vienna, Austria.

Cell
|September 3, 2005
PubMed
概括

在Drosophila中,一种新的细胞命机制使用循环内体来控制Notch/Delta信号,独立于Numb和Neuralized. 这一途径通过影响内细胞区形成来调节发育命运.

更多相关视频

Retrograde Tracing of Drosophila Embryonic Motor Neurons Using Lipophilic Fluorescent Dyes
08:25

Retrograde Tracing of Drosophila Embryonic Motor Neurons Using Lipophilic Fluorescent Dyes

Published on: January 13, 2020

Antibody Uptake Assay for Tracking Notch/Delta Endocytosis During the Asymmetric Division of Zebrafish Radial Glia Progenitors
09:06

Antibody Uptake Assay for Tracking Notch/Delta Endocytosis During the Asymmetric Division of Zebrafish Radial Glia Progenitors

Published on: January 20, 2023

相关实验视频

Last Updated: Jul 1, 2026

Live-cell Imaging of Sensory Organ Precursor Cells in Intact Drosophila Pupae
04:27

Live-cell Imaging of Sensory Organ Precursor Cells in Intact Drosophila Pupae

Published on: May 27, 2011

Retrograde Tracing of Drosophila Embryonic Motor Neurons Using Lipophilic Fluorescent Dyes
08:25

Retrograde Tracing of Drosophila Embryonic Motor Neurons Using Lipophilic Fluorescent Dyes

Published on: January 13, 2020

Antibody Uptake Assay for Tracking Notch/Delta Endocytosis During the Asymmetric Division of Zebrafish Radial Glia Progenitors
09:06

Antibody Uptake Assay for Tracking Notch/Delta Endocytosis During the Asymmetric Division of Zebrafish Radial Glia Progenitors

Published on: January 20, 2023

科学领域:

  • 细胞生物学 细胞生物学
  • 发展生物学 发展生物学
  • 分子生物学分子生物学

背景情况:

  • 果虫感官器官前体 (SOP) 细胞表现出不对称的细胞分裂.
  • 麻木和神经化通常通过影响子细胞中的Notch/Delta信号来调节细胞命运.

研究的目的:

  • 在细胞酸盐规范中识别Numb和Neuralized独立机制.
  • 为了研究内细胞贩运在调节细胞命运中的作用在多索菲拉发育过程中.

主要方法:

  • 使用Drosophila melanogaster作为一个模型生物.
  • 使用细胞培养系统 (包括哺乳动物) 来研究内细胞通路.
  • 研究了蛋白质局部化和相互作用,专注于Rab 11和核沉降.

主要成果:

  • 发现了一种独立于Numb和Neuralized的细胞酸盐规范的新机制.
  • 通过在子细胞中的循环内分体 (Rab 11-阳性) 证明了诺奇配方Delta的差异性贩运.
  • 表明,在一个子细胞 (pIIa) 中不能形成循环内分体,是由于核沉降对中心细胞的招募受损.
  • 通过使用哺乳动物细胞培养,证实了循环内分体在三角洲活动中的重要作用.

结论:

  • 细胞命运可以通过调节特定内细胞区的形成来确定.
  • 循环内分泌体的形成对Notch/Delta信号通路活动至关重要.
  • 这项研究揭示了不对称细胞分裂和发育信号的新层调节.