不对称的Rab 11内基因组调节三角形循环,并确定Drosophila神经系统中的细胞命运
Gregory Emery1, Andrea Hutterer, Daniela Berdnik
1Institute of Molecular Biotechnology of the Austrian Academy of Sciences, Dr. Bohr Gasse 3-5, 1030 Vienna, Austria.
Cell
|September 3, 2005
概括
在Drosophila中,一种新的细胞命机制使用循环内体来控制Notch/Delta信号,独立于Numb和Neuralized. 这一途径通过影响内细胞区形成来调节发育命运.
科学领域:
- 细胞生物学 细胞生物学
- 发展生物学 发展生物学
- 分子生物学分子生物学
背景情况:
- 果虫感官器官前体 (SOP) 细胞表现出不对称的细胞分裂.
- 麻木和神经化通常通过影响子细胞中的Notch/Delta信号来调节细胞命运.
研究的目的:
- 在细胞酸盐规范中识别Numb和Neuralized独立机制.
- 为了研究内细胞贩运在调节细胞命运中的作用在多索菲拉发育过程中.
主要方法:
- 使用Drosophila melanogaster作为一个模型生物.
- 使用细胞培养系统 (包括哺乳动物) 来研究内细胞通路.
- 研究了蛋白质局部化和相互作用,专注于Rab 11和核沉降.
主要成果:
- 发现了一种独立于Numb和Neuralized的细胞酸盐规范的新机制.
- 通过在子细胞中的循环内分体 (Rab 11-阳性) 证明了诺奇配方Delta的差异性贩运.
- 表明,在一个子细胞 (pIIa) 中不能形成循环内分体,是由于核沉降对中心细胞的招募受损.
- 通过使用哺乳动物细胞培养,证实了循环内分体在三角洲活动中的重要作用.
结论:
- 细胞命运可以通过调节特定内细胞区的形成来确定.
- 循环内分泌体的形成对Notch/Delta信号通路活动至关重要.
- 这项研究揭示了不对称细胞分裂和发育信号的新层调节.
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