转录基因组中小RNA组件的解
Cheng Lu1, Shivakundan Singh Tej, Shujun Luo
1Delaware Biotechnology Institute, University of Delaware, Newark, DE 19711, USA.
概括
研究人员从Arabidopsis thaliana中鉴定出超过75,000个小RNA序列,包括微RNA (miRNA) 和小干扰RNA (siRNA). 许多以前被认为没有特征的基因组区域被发现产生了大量的小RNA.
科学领域:
- 植物分子生物学 植物分子生物学
- 基因组学就是基因组学.
- RNA生物学的RNA生物学
背景情况:
- 小RNA是真核生物中关键的调节分子,但许多仍未确定.
- 了解小RNA的多样性和起源对于破译基因调节至关重要.
研究的目的:
- 为了全面识别和描述模型植物Arabidopsis thaliana中的小RNA.
- 研究不同小RNA类的丰富性和基因组起源.
主要方法:
- 来自Arabidopsis thaliana幼苗和花朵的200多万个小RNA分子的高通量测序.
- 生物信息分析用于识别已知和新型的微RNA (miRNA) 和小干扰RNA (siRNA).
主要成果:
- 确定了超过75,000个独特的小RNA序列.
- 微RNAs (miRNAs) 非常丰富,还有大量的小干扰RNAs (siRNAs) 映射到重复元素,基因间区域和基因.
- 检测到许多小RNA来自以前未被描述的基因组区域.
结论:
- 阿拉比多普西斯塔利亚纳拥有庞大而多样化的小RNA种群.
- 小干扰RNAs (siRNAs) 是普遍存在的,并来自广泛的基因组位置.
- 这项研究扩大了植物中小RNA生产的已知景观.
相关概念视频
RNA Interference
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
RNA Interference
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
siRNA - Small Interfering RNAs
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
lncRNA - Long Non-coding RNAs
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA (lncRNA)...
Small interfering RNAs (siRNA)
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
lncRNA - Long Non-coding RNAs
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA (lncRNA)...


