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13:58
Peptide:MHC Tetramer-based Enrichment of Epitope-specific T cells
Published on: October 22, 2012
用于向MHCII类受限制T细胞呈现细胞质抗原的处理途径
M S Malnati1, M Marti, T LaVaute
1Laboratory of Immunogenetics, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, Maryland 20852.
Nature
|June 25, 1992
概括
辅助T细胞通过HLA-DR1识别细胞溶解抗原,即使没有抗原呈现的载体. 这种产生T细胞表位的途径不同于经典的I类途径.
科学领域:
- 免疫学 免疫学 免疫学
- 分子生物学分子生物学
背景情况:
- CD4+ T 细胞抗原通常来自外部蛋白质,这些蛋白质在内细胞中被加工,由MHC II 类分子呈现.
- CD8+ T 细胞抗原通常来源于细胞质蛋白质,载入MHC I 类,取决于抗原呈现 (TAP) 的载体.
- 内生细胞质抗原也可以由MHCII类分子呈现,这表明了替代的抗原处理途径.
研究的目的:
- 研究细胞抗原的HLA-DR1受限识别机制.
- 确定是否需要抗原呈现载体 (TAP) 来向CD4+T细胞呈现内源性细胞质抗原.
- 阐明从细胞溶解抗原中帮助T细胞表皮质生成的途径.
主要方法:
- 使用了缺乏抗原呈现载体 (TAP) 的突变细胞系.
- 在这些突变细胞中评估了细胞质抗原的HLA-DR1受限制的T细胞识别.
- 研究了TAP在向DR1-受限制的T细胞呈现短细胞质中的作用.
主要成果:
- 复杂细胞质抗原的HLA-DR1受限识别发生在缺乏TAP的细胞中.
- 由DR1-受限制的T细胞识别一个短的细胞解质取决于TAP.
- 证明了细胞质抗原对CD4+T细胞的TAP独立呈现是可能的.
结论:
- 辅助T细胞表位可以通过多种机制从细胞溶解抗原生成.
- 通过HLA-DR1向CD4+T细胞呈现细胞质抗原的一种机制是独立于抗原呈现载体 (TAP).
- 与经典的I类抗原呈现途径相比,这种TAP独立的途径代表了一个独特的机制.
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