对于Ly-49+IL-2-激活自然杀手细胞的MHC I类异抗原特异性
F M Karlhofer1, R K Ribaudo, W M Yokoyama
1Rosalind Russell Arthritis Research Laboratory, Department of Medicine, University of California, San Francisco.
自然杀手 (NK) 细胞使用Ly-49受体来识别特定的细胞. Ly-49+ NK 细胞抵御了表达某些主要基因相容性复合体 (MHC) I 类抗原的化细胞,这表明了特定的抑制相互作用.
科学领域:
- 免疫学 免疫学 免疫学
- 细胞和分子免疫学 细胞和分子免疫学
背景情况:
- 通过CD3-自然杀手 (NK) 细胞识别细胞的分子机制尚未完全理解.
- 主体组织相容性复合体 (MHC) 目标细胞上的I类表达通常与对NK细胞介导溶解的抵抗相关,这表明抑制信号传递.
- NK细胞全特异性涉及MHC I类结裂,为了解NK细胞识别提供了潜在的途径.
研究的目的:
- 研究Ly-49分子在NK细胞对目标细胞的识别中的作用.
- 阐明NK细胞全特异性和抑制信号的分子基础.
主要方法:
- 从表达Ly-49的C57BL/6小鼠中利用了介质素-2-激活的NK细胞.
- 评估了NK细胞介导的化目标细胞,具有不同的MHC I类抗原表达 (H-2d,H-2k).
- 采用基因转染来在细胞上表达特定的MHC I类分子 (H-2Dd,H-2Kd,H-2Ld),并使用单克隆抗体来阻止相互作用.
主要成果:
- 与Ly-49-NK细胞不同的是,Ly-49+NK细胞对表达H-2d或H-2k的细胞的溶解有抗性.
- 传染H-2Dd,但不是H-2Kd或H-2Ld,使敏感的点细胞对Ly-49+NK细胞产生抗性.
- 抗体介导的Ly-49或H-2Dd的α1/α2域的阻断取消了这种抵抗,表明了特定的相互作用.
结论:
- Ly-49+ NK 细胞与MHC I 类异抗原H-2Dd.的结域特别相互作用.
- 这些发现表明NK细胞具有抑制受体,可以识别特定的MHC I类抗原,从而有助于细胞的识别.
更多相关视频
10:01Radial Mobility and Cytotoxic Function of Retroviral Replicating Vector Transduced, Non-adherent Alloresponsive T Lymphocytes
Published on: February 11, 2015
06:55Measurement of Natural Killer Cell-Mediated Cytotoxicity and Migration in the Context of Hepatic Tumor Cells
Published on: February 22, 2020
相关概念视频
Cells of the Innate Immune Response
Phagocytes
Phagocytes police the peripheral tissues by removing cellular debris and responding to the invasion of foreign substances or pathogens. Many phagocytes attack and remove microorganisms even before lymphocytes detect them. The human body has two general...
Antigens Involved in Adaptive Immunity
Complete Antigens
Complete antigens possess both immunogenicity and reactivity.
Antigen Processing Pathways
MHC Class I: Presenting Endogenous...
T Cell Activation and Clonal Selection
Naive T cells that have not yet encountered an antigen express two primary CD...
Cytotoxic T Cells-mediated Immune Response
Immunological surveillance is the ability of immune cells to monitor and eliminate infected cells with intracellular pathogens, neoplastically transformed cells, and cells with non-self antigens. Cytotoxic T cells and NK...
Immune Response Against Viral Pathogens
NK Cells
NK cells are a crucial part of our innate immune system, acting as the first line of defense against viral infections. These cells can recognize and kill infected cells without prior exposure to the virus, effectively slowing down the spread of infection. Additionally, NK cells produce proinflammatory...
