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石格拉柔性菌诱导受感染的巨细胞的亡
A Zychlinsky1, M C Prevost, P J Sansonetti
1Unité de Pathogénie Microbienne Moléculaire, INSERM U199, Institut Pasteur, Paris, France.
Nature
|July 9, 1992
概括
湿疹病原体Shigella flexneri在宿主巨细胞中诱导编程细胞死亡 (细胞亡). 这种由细菌诱导的宿主细胞自杀对其入侵和病变产生至关重要.
科学领域:
- 微生物学 微生物学
- 细胞生物学 细胞生物学
- 病变的发生和发病.
背景情况:
- 西格拉柔性菌通过入侵结肠粘膜来引起痢疾.
- 病原体侵入肠细胞,进入细胞质,并扩散到邻近的细胞.
- 希格拉菌到达自身膜,在那里它遇到并杀死细胞,导致形成.
研究的目的:
- 阐明Shigella flexneri对巨细胞的细胞毒性机制.
- 为了确定细菌入侵是否与诱导编程细胞死亡有关.
主要方法:
- 测试四种不同的Shigella flexneri菌株,以检测它们诱导细胞死亡的能力.
- 将一种侵入性菌株与其非侵入性,用等离子体治疗的同源菌株进行比较.
- 分析paB (入侵蛋白抗原) 中的一种突变和表达大肠杆菌血溶素操作子的菌株.
主要成果:
- 一种入侵性Shigella flexneri菌株诱导了巨细胞的编程细胞死亡 (细胞亡).
- 一种非侵入性,用等离子体治愈的菌株和一种IpaB突变体是无毒的.
- 一种表达大肠杆菌血清酶的非侵入性菌株诱导了亡,而不是亡.
结论:
- 作为细胞毒性的机制,Shigella flexneri积极诱导宿主巨细胞的亡.
- 这是第一个侵入性细菌病原体诱导宿主细胞自杀的证据.
- 细菌入侵和特定的毒性因素对于诱导亡至关重要.
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