Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
Regulation of Metabolism01:19

Regulation of Metabolism

Cellular needs and conditions vary from cell to cell and change within individual cells over time. For example, the required enzymes and energetic demands of stomach cells are different from those of fat storage cells, skin cells, blood cells, and nerve cells. Furthermore, a digestive cell works much harder to process and break down nutrients during the time that closely follows a meal compared with many hours after a meal. As these cellular demands and conditions vary, so do the amounts and...
PI3K/mTOR/AKT Signaling Pathway01:22

PI3K/mTOR/AKT Signaling Pathway

The mammalian target of rapamycin  (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1  (mTORC1) and mTOR complex 2  (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast,  mTORC2 consists of a rapamycin-insensitive companion...
cAMP-dependent Protein Kinase Pathways01:25

cAMP-dependent Protein Kinase Pathways

Cyclic Adenosine Monophosphate (cAMP) is an essential second messenger that activates protein kinase A (PKA) and regulates various biological processes. A single epinephrine molecule binds to GPCR and activates several heterotrimeric G proteins, each stimulating multiple adenylyl cyclase, amplifying the signal, and synthesizing large numbers of cAMP molecules. Small changes in cAMP concentration affect PKA activity. The binding of four cAMP molecules induces a conformational change in PKA,...
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
Regulation of Food Intake01:30

Regulation of Food Intake

Short-term regulation of food intake primarily involves neural signals from the gastrointestinal (GI) tract, blood nutrient levels, and GI tract hormones. Communication between the gut and brain via vagal nerve fibers plays a significant role in evaluating the contents of the gut. Clinical studies have shown that protein ingestion produces a more prolonged response in these nerve fibers compared to an equivalent amount of glucose. Additionally, the activation of stretch receptors caused by GI...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Schisandrin B exhibits anti‑proliferative effects by inducing ferroptosis in pancreatic cancer.

Oncology reports·2026
Same author

Enhanced <i>in vitro</i> photodynamic performance under hypoxia-related conditions by BP-Au@MnO<sub>2</sub><b>-</b>Ce6 nanocomposites.

Journal of materials chemistry. B·2026
Same author

A molecular switch in NAC prevents mitochondrial protein mistargeting by SRP.

Nature communications·2026
Same author

Destabilized Soluble SOD1 Species as Potential Determinants of Disease Severity in Familial Amyotrophic Lateral Sclerosis.

ACS chemical neuroscience·2026
Same author

Functional rescue of a disease-linked ERAD pathway mutation via alternative splicing.

The EMBO journal·2026
Same author

SEL1L-HRD1 ERAD-autophagy interplay maintains mitochondrial homeostasis in brown adipocytes.

Proceedings of the National Academy of Sciences of the United States of America·2026

相关实验视频

Updated: Jul 5, 2026

High-resolution Respirometry to Measure Mitochondrial Function of Intact Beta Cells in the Presence of Natural Compounds
12:32

High-resolution Respirometry to Measure Mitochondrial Function of Intact Beta Cells in the Presence of Natural Compounds

Published on: January 23, 2018

克雷布联合激活剂TORC2是禁食葡萄糖代谢的关键调节剂.

Seung-Hoi Koo1, Lawrence Flechner, Ling Qi

  • 1Peptide Biology Laboratories, Salk Institute for Biological Studies, 10010 N Torrey Pines Rd, La Jolla, California 92037-1002, USA.

Nature
|September 9, 2005
PubMed
概括

荷尔蒙和能量信号汇聚在TORC2 (调节CREB活动2的传感器) 上,以控制肝脏葡萄糖的产生. AMPK激活通过阻止TORC2核进入来抑制葡萄糖生成,提供潜在的2型糖尿病疗法.

更多相关视频

A Flexible Low Cost Hydroponic System for Assessing Plant Responses to Small Molecules in Sterile Conditions
11:27

A Flexible Low Cost Hydroponic System for Assessing Plant Responses to Small Molecules in Sterile Conditions

Published on: August 25, 2018

Isolation of Primary Mouse Hepatocytes for Nascent Protein Synthesis Analysis by Non-radioactive L-azidohomoalanine Labeling Method
08:04

Isolation of Primary Mouse Hepatocytes for Nascent Protein Synthesis Analysis by Non-radioactive L-azidohomoalanine Labeling Method

Published on: October 23, 2018

相关实验视频

Last Updated: Jul 5, 2026

High-resolution Respirometry to Measure Mitochondrial Function of Intact Beta Cells in the Presence of Natural Compounds
12:32

High-resolution Respirometry to Measure Mitochondrial Function of Intact Beta Cells in the Presence of Natural Compounds

Published on: January 23, 2018

A Flexible Low Cost Hydroponic System for Assessing Plant Responses to Small Molecules in Sterile Conditions
11:27

A Flexible Low Cost Hydroponic System for Assessing Plant Responses to Small Molecules in Sterile Conditions

Published on: August 25, 2018

Isolation of Primary Mouse Hepatocytes for Nascent Protein Synthesis Analysis by Non-radioactive L-azidohomoalanine Labeling Method
08:04

Isolation of Primary Mouse Hepatocytes for Nascent Protein Synthesis Analysis by Non-radioactive L-azidohomoalanine Labeling Method

Published on: October 23, 2018

科学领域:

  • 代谢调节 代谢调节 代谢调节
  • 荷尔蒙信号传递方式
  • 感知细胞能量的细胞能量.

背景情况:

  • 葡萄糖平衡依赖于全身激素 (胰岛素,葡萄糖) 和细胞能量状态.
  • 葡萄糖激素通过激活CREB (cAMP反应元素结合蛋白) 来促进肝脏的葡萄糖输出.
  • 在低ATP的情况下,AMP激活蛋白激酶 (AMPK) 抑制肝脏葡萄糖生成,但机制尚不清楚.

研究的目的:

  • 阐明AMPK抑制肝脏葡萄糖生成的机制.
  • 确定在葡萄糖调节中的荷尔蒙和能量传感途径的融合点.
  • 调查TORC2 (调节CREB活动2的传感器) 在调节葡萄糖输出中的作用.

主要方法:

  • 研究了AMPK,TORC2和CREB信号之间的相互作用.
  • 利用细胞和分子生物学技术来追踪TORC2的定位和活动.
  • 研究了AMPK激活对TORC2酸化和核转位的影响.

主要成果:

  • 荷尔蒙和能量感应通路汇聚在同活性器TORC2.2.上.
  • TORC2在细胞质和细胞核之间穿,调节糖原基因的CREB-依赖转录.
  • AMPK激活促进TORC2酸化,防止其核积累,并抑制葡萄糖生成.

结论:

  • 在肝脏葡萄糖生产过程中,TORC2作为激素和能量信号的关键集成者.
  • 通过AMPK介导的TORC2核进口的抑制是抑制葡萄糖生成的关键机制.
  • 向TORC2酸化可能代表一种治疗策略,用于管理2型糖尿病的禁食高血糖症.