在植物细胞中Agrobacterium VirE2蛋白的核定位
V Citovsky1, J Zupan, D Warnick
1Department of Plant Biology, University of California, Berkeley 94720.
概括
亚格罗细菌VirE2蛋白质有助于将T链DNA转移到植物核中. 这种对瘤形成至关重要的蛋白质含有核定位信号 (NLS),并促进DNA导入.
科学领域:
- 分子生物学分子生物学
- 植物病理学 植物病理学
- 微生物遗传学微生物遗传学
背景情况:
- 阿格罗细菌tumefaciens将T链DNA转移到植物细胞中.
- T-链是一种单链DNA (ssDNA) 的中间体.
- 维尔D2蛋白与T链的5'端结合,而维尔E2覆盖了DNA.
研究的目的:
- 调查VirE2在AgrobacteriumT-strand的核进口中的作用.
- 确定VirE2是否拥有核定位信号 (NLS) 并有助于核吸收.
主要方法:
- 将VirE2与记者蛋白融合,以追踪其在植物细胞中的定位.
- 在VirE2中对氨基酸序列进行分析,以获得已知NLS的同质性.
- 使用virE2突变体和表达VirE2.2的转基因植物的补充试验.
主要成果:
- 维尔E2与植物细胞核局部化的记者蛋白融合.
- 维尔E2 包含两个同源于Xenopus核氨酸的双部分NLS的序列.
- 转基因植物中VirE2的表达恢复了病毒性virE2突变的瘤性.
结论:
- 在植物细胞内T链DNA的核进口中,VirE2起着至关重要的作用.
- VirE2拥有功能性的NLS,调解自己的核定位,可能有助于T链核吸收.
- 维尔E2对于Agrobacterium介导的植物转化和植物中的瘤发生至关重要.
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