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相关概念视频

COP Coated Vesicles00:59

COP Coated Vesicles

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Membrane-enclosed structures called vesicles transport proteins and lipids across the cell. The vesicles derive their cargo from the plasma membrane, Golgi, ER, or endosome. Coated vesicles are spherical, protein-coated carriers with a 50–100 nm diameter that mediate bidirectional transport between the ER and the Golgi. The distribution of proteins between the ER and Golgi complex is dynamic and is maintained by different coated vesicles. Their formation is driven by the assembly of...
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Vesicle budding is orchestrated by distinct cytosolic proteins such as adaptor proteins, coat proteins, and GTPases. To initiate vesicle budding, membrane-bending proteins containing crescent-shaped BAR domains bind to the lipid heads in the bilayer and distort the membrane to form a protein-coated vesicle bud. Adaptors proteins such as AP2 for clathrin-coated vesicles can nucleate on the deformed membrane. Finally, coat proteins such as clathrin or COPI and COPII assemble into a coat forming...
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Rab Proteins01:14

Rab Proteins

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Rab proteins constitute the largest family of monomeric GTPases, of which 70 members are present in humans. Rab proteins and their effectors regulate consecutive stages of vesicle transport such as vesicle transport, docking, and fusion to the correct recipient membrane.
Rab proteins switch between a cytosolic, GDP-bound inactive state and a membrane-anchored, GTP-bound active state. By themselves, Rabs show slow rates of GDP/GTP exchange and GTP hydrolysis. Thus, Rab proteins are considered...
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Overview of Secretory Vesicles01:33

Overview of Secretory Vesicles

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Secretory vesicles, also known as dense core vesicles (DCVs), are membrane-bound vesicles that transport secretory proteins, such as hormones or neurotransmitters. Regulated secretory vesicles transport proteins from the trans-Golgi network to the exterior of the cell. Proteins present in regulated secretory vesicles are required to be rapidly exocytosed in large amounts upon a specific stimulus.
Various proteins regulate the aggregation of molecules inside the secretory vesicles. Chromogranins...
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Fusion of Secretory Vesicles with the Plasma Membrane01:26

Fusion of Secretory Vesicles with the Plasma Membrane

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Proteins and neurotransmitters in secretory vesicles can be released from a cell upon vesicle docking, priming, and fusion with the plasma membrane. Vesicles are docked and primed in preparation for the quick exocytosis of their contents in response to a stimulus. The fusion process is mainly carried out by a SNAP Receptor or SNARE complex, consisting of synaptobrevin, syntaxin-1, and SNAP-25.
In 1993, Jim Rothman proposed that the antiparallel pairing of vesicular and transmembrane SNAREs, or...
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相关实验视频

Updated: Feb 25, 2026

Directed Assembly of Elastin-like Proteins into defined Supramolecular Structures and Cargo Encapsulation In Vitro
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基于水解性自我交叉链接的共聚合物的pH响应囊泡.

Jianzhong Du1, Steven P Armes

  • 1Department of Chemistry, The University of Sheffield, Brook Hill, Sheffield S3 7HF, UK.

Journal of the American Chemical Society
|September 15, 2005
PubMed
概括

研究人员使用独特的共聚合物开发了新的pH响应囊泡. 这些自组装,交叉连接的囊泡表现出可调节的透性,为先进的材料应用提供了潜力.

科学领域:

  • 聚合物化学 聚合物化学
  • 材料科学 材料科学 材料科学
  • 纳米技术 纳米技术

背景情况:

  • 响应刺激的材料对于先进的应用至关重要.
  • 囊泡为封装和输送提供多功能平台.
  • 控制囊泡形态和稳定性是它们有用性的关键.

研究的目的:

  • 为了合成和表征新的形状持久,pH响应的囊泡.
  • 为了研究一个特定的共聚合物的自我组装和交联行为.
  • 评估准备好的囊泡的pH敏感性和功能化潜力.

主要方法:

  • 聚乙烯氧化物-块聚二乙烯甲酸-国家三三氧化) 甲酸) (PEO-b-P) (DEA-stat-TMSPMA)) 合聚合物的自组.
  • 囊泡膜壁的水解性交叉连接.
  • 使用1H NMR,TEM,DLS和停止流的光进行表征.
  • 在囊泡壁内的金纳米颗粒的现场合成.

主要成果:

  • 在水溶液中自发形成稳定,形状持久的囊泡.
  • 证明了囊泡壁的pH响应透性.
  • 成功地将金纳米颗粒纳入囊泡膜.

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  • PEO链形成了冠状,而P ((DEA-stat-TMSPMA) 形成了pH敏感的壁.

    • 结论:

    • 新的PEO-b-P ((DEA-stat-TMSPMA) 共聚合物使得能够创建强大的,对pH响应的囊泡.
    • 水解性交叉连接策略有效地稳定了囊泡形态.
    • 对pH值敏感的透性和金纳米粒子装饰突出了药物输送和传感方面的潜在应用.