胚胎干细胞免疫性在移植到缺血性心肌之后的分化后增加
Rutger-Jan Swijnenburg1, Masashi Tanaka, Hannes Vogel
1Department of Surgery, Leiden University School of Medicine, The Netherlands. rjswijnenburg@hotmail.com
Circulation
|September 15, 2005
概括
心脏中的分化胚胎干细胞 (ESC) 引发更快的免疫反应,增加排斥风险. 这表明,用于心脏修复的全源ESC可能需要免疫抑制.
科学领域:
- 心脏病学 心脏病学
- 免疫学 免疫学 免疫学
- 再生医学是一种再生医学.
背景情况:
- 研究缺血性心肌内分化的胚胎干细胞 (ESC) 的免疫性.
- 在心脏环境中评估ESC移植后免疫排斥的可能性.
研究的目的:
- 为了确定ESCs在缺血性心脏组织中的体内分化是否增强了它们的免疫性.
- 在心脏移植模型中,评估差异化ESC与无差异化ESC的免疫反应和排斥率.
主要方法:
- 鼠标ESCs (ES-D3线) 被移植到肌心梗塞后的异构和异构小鼠心脏中.
- 免疫组织化学被用来分析免疫细胞透和ESC分化在不同的时间点.
- 第二个系列涉及ESC注射心脏的异型移植,以评估免疫反应.
主要成果:
- 在2周后,ESC移植没有表现出差异化,但在4周后形成了瘤.
- 在8周内检测到同源性ESC,而异源性ESC则没有.
- 从一周开始,全物接受者表现出炎症细胞透率的增加,在4-8周内变得充满活力.
结论:
- 在体内分化的ESCs比未分化的ESCs引起更快的免疫反应.
- 在临床上使用异性ESC或其衍生品治疗心力衰竭可能需要免疫抑制策略.
相关概念视频
01:17Stem Cell Culture
Stem cell research aims to find ways to use stem cells to regenerate and repair cellular damage. Over time, most adult cells undergo the wear and tear of aging and lose their ability to divide and repair themselves. Stem cells do not display a particular morphology or function. Adult stem cells, which exist as a small subset of cells in most tissues, keep dividing and can differentiate into a number of specialized cells generally formed by that tissue. These cells enable the body to renew and...
01:22iPS Cell Differentiation
The ability of induced pluripotent stem cells or iPSCs to differentiate into most body cell types has stimulated repair and regenerative medicine research over the past few decades. iPSC-derived blood cells, hepatocytes, beta islet cells, cardiomyocytes, neurons, and other cell types can repair injuries or regenerate damaged tissue in diseases such as diabetes and neurodegenerative disorders.