矩阵嵌入改变了对内皮细胞的免疫反应 in vitro 和 in vivo
Heiko Methe1, Helen M Nugent, Adam Groothuis
1Massachusetts Institute of Technology, Cambridge, MA 02139, USA. hmethe@mit.edu
Circulation
|September 15, 2005
概括
将内皮细胞 (EC) 嵌入3D矩阵中可以减少免疫排斥. 这种策略抑制了免疫分子的表达,为血管疾病治疗提供了一种新方法.
科学领域:
- 血管生物学 血管生物学
- 免疫学 免疫学 免疫学
- 生物材料科学 生物材料科学
背景情况:
- 内皮细胞 (EC) 功能障碍是动脉样硬化的早期迹象.
- 由于局部炎症和改变的血液流动,恢复内皮是具有挑战性的.
- 周血管EC矩阵植入物提供保护和控制血管修复,但其免疫反应是未知的.
研究的目的:
- 研究将EC嵌入3D矩阵对宿主免疫反应的影响.
- 评估Gelfoam/EC植入物在体外和体内免疫调节作用.
主要方法:
- 使用流细胞计对自由与嵌入式EC的MHC,共刺激和粘附分子表达进行了比较.
- 通过 [3H] 胺素的结合来评估T细胞的增殖.
- 在小鼠体内注射自由ECs,Gelfoam/EC块或与Gelfoam相邻的ECs后,研究了小鼠体内和细胞免疫反应.
主要成果:
- 在Gelfoam矩阵中的EC显示MHCII类,共刺激和粘附分子的表达明显较低.
- 与自由EC相比,嵌入式EC诱导的T细胞增殖率是自由EC的3倍.
- 在老鼠身上植入的Gelfoam/EC植入物几乎取消了免疫反应,减少了炎症标志物和抗体生产的1.75至9.0倍.
结论:
- 在3D矩阵中嵌入EC可以显著改变宿主免疫反应.
- 这种方法抑制了关键的免疫分子表达,表明了新型血管疾病治疗的潜力.
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