膜囊泡传递交通信号,并促进 prokaryote 的群体活动
Lauren M Mashburn1, Marvin Whiteley
1Department of Periodontics, The University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma 73104, USA.
Nature
|September 16, 2005
概括
伪 aeruginosa 使用膜囊泡来传输象限检测信号,如伪 quinolone 信号 (PQS). 这种囊泡介导的系统对于细菌的交流和协调的群体行为至关重要.
科学领域:
- 微生物学 微生物学
- 细菌的传播 细菌的传播
- 分子生物学分子生物学
背景情况:
- 细菌通过定数感应利用细胞外信号协调群体行为.
- 对于疏水性定数感应分子的贩运机制,比如 Pseudomonas quinolone signal (PQS),目前尚不清楚.
- Pseudomonas aeruginosa 是一种机会性病原体,在这种病原体中,定数感应起着至关重要的作用.
研究的目的:
- 阐明 Pseudomonas aeruginosa 贩卖定数感应分子 PQS. 的机制.
- 研究膜囊泡在细菌群体内的PQS传输中的作用.
- 了解PQS包装如何影响协调群体行为.
主要方法:
- 利用基因操纵从 Pseudomonas aeruginosa 种群中去除膜囊泡.
- 量化了PQS水平和评估了细菌群体在囊泡存在或不存在时的行为.
- 调查了PQS在自身囊泡包装和相关抗微生物类素包装中的作用.
主要成果:
- Pseudomonas aeruginosa 将信号分子 PQS 包装成膜囊泡,用于细胞间的贩运.
- 移除这些囊泡会破坏细胞之间的通信,并抑制PQS控制的群体行为.
- PQS积极调解其自身的包装和其他抗微生物醇的包装到囊泡中.
结论:
- Prokaryotes 拥有复杂的信号传输系统,类似于高级生物中的信号传输系统,用于定数感应分子.
- 膜囊泡代表了一种新的机制,用于传递协调P. aeruginosa.群体行为的关键信号.
- 这一发现为细菌的社会相互作用和毒性策略提供了新的见解.
相关概念视频
01:33Overview of Secretory Vesicles
Secretory vesicles, also known as dense core vesicles (DCVs), are membrane-bound vesicles that transport secretory proteins, such as hormones or neurotransmitters. Regulated secretory vesicles transport proteins from the trans-Golgi network to the exterior of the cell. Proteins present in regulated secretory vesicles are required to be rapidly exocytosed in large amounts upon a specific stimulus.
Various proteins regulate the aggregation of molecules inside the secretory vesicles. Chromogranins...
Various proteins regulate the aggregation of molecules inside the secretory vesicles. Chromogranins...
01:44Introduction to Membrane Traffic
The ER, Golgi apparatus, endosomes, and lysosomes work in tandem to modify, sort, and package proteins and lipids. An integrated membrane trafficking network facilitates the back and forth shuttling of molecules within different organelles in the same cell or across the cell membrane.
The transport of soluble and membrane proteins is mediated by transport vesicles that collect cargo from one cellular compartment and deliver it to another by fusing with the target organelle membrane. The Rab...
The transport of soluble and membrane proteins is mediated by transport vesicles that collect cargo from one cellular compartment and deliver it to another by fusing with the target organelle membrane. The Rab...
00:59COP Coated Vesicles
Membrane-enclosed structures called vesicles transport proteins and lipids across the cell. The vesicles derive their cargo from the plasma membrane, Golgi, ER, or endosome. Coated vesicles are spherical, protein-coated carriers with a 50–100 nm diameter that mediate bidirectional transport between the ER and the Golgi. The distribution of proteins between the ER and Golgi complex is dynamic and is maintained by different coated vesicles. Their formation is driven by the assembly of different...
01:43SNAREs and Membrane Fusion
Once a transport vesicle has recognized its target organelle, the vesicular membrane needs to fuse with the target membrane to unload the cargo. Transmembrane proteins called SNAREs present on organelle membranes and their vesicles, mediate vesicle fusion.
SNAREs exist in pairs that symmetrically interact and catalyze the fusion of the lipid bilayers in vesicle and target organelle. v-SNARE in the vesicle membrane are single polypeptide chains that bind to a complementary t-SNARE, composed of 2...
SNAREs exist in pairs that symmetrically interact and catalyze the fusion of the lipid bilayers in vesicle and target organelle. v-SNARE in the vesicle membrane are single polypeptide chains that bind to a complementary t-SNARE, composed of 2...
01:45Vesicular Tubular Clusters
After budding out from the ER membrane, some COPII vesicles lose their coat and fuse with one another to form larger vesicles and interconnected tubules called vesicular tubular clusters or VTCs. These clusters constitute a compartment at the ER-Golgi interface known as ERGIC (Endoplasmic Reticulum Golgi Intermediate Compartment). The ERGIC is a mobile membrane-bound cargo transport system that sorts proteins secreted from ER and delivers them to the Golgi.
With the help of motor proteins such...
With the help of motor proteins such...
01:44Introduction to Membrane Traffic
The ER, Golgi apparatus, endosomes, and lysosomes work in tandem to modify, sort, and package proteins and lipids. An integrated membrane trafficking network facilitates the back and forth shuttling of molecules within different organelles in the same cell or across the cell membrane.
The transport of soluble and membrane proteins is mediated by transport vesicles that collect cargo from one cellular compartment and deliver it to another by fusing with the target organelle membrane. The Rab...
The transport of soluble and membrane proteins is mediated by transport vesicles that collect cargo from one cellular compartment and deliver it to another by fusing with the target organelle membrane. The Rab...