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相关概念视频

Co-activators and Co-repressors02:04

Co-activators and Co-repressors

Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
NF-κB-dependent Signaling Pathway02:26

NF-κB-dependent Signaling Pathway

The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The heterodimer of NF-κB...
GPCRs Regulate Adenylyl Cylase Activity01:09

GPCRs Regulate Adenylyl Cylase Activity

Some GPCRs transmit signals through adenylyl cyclase (AC), a transmembrane enzyme. AC helps synthesize second messenger cyclic adenosine monophosphate (cAMP). AC catalyzes cyclization reaction and converts ATP to cAMP by releasing a pyrophosphate. The pyrophosphate is further hydrolyzed to phosphate by the enzyme pyrophosphatase, which drives cAMP synthesis to completion. However, cAMP is rapidly degraded to 5′ AMP by the enzymes phosphodiesterase (PDE), preventing overstimulation of cells.
Two...
cAMP-dependent Protein Kinase Pathways01:25

cAMP-dependent Protein Kinase Pathways

Cyclic Adenosine Monophosphate (cAMP) is an essential second messenger that activates protein kinase A (PKA) and regulates various biological processes. A single epinephrine molecule binds to GPCR and activates several heterotrimeric G proteins, each stimulating multiple adenylyl cyclase, amplifying the signal, and synthesizing large numbers of cAMP molecules. Small changes in cAMP concentration affect PKA activity. The binding of four cAMP molecules induces a conformational change in PKA,...
NF-kB-dependent Signaling Pathway02:26

NF-kB-dependent Signaling Pathway

The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The heterodimer of NF-κB...

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相关实验视频

Updated: Jul 16, 2026

Synthesis and Characterization of an Aspirin-fumarate Prodrug that Inhibits NFκB Activity and Breast Cancer Stem Cells
13:38

Synthesis and Characterization of an Aspirin-fumarate Prodrug that Inhibits NFκB Activity and Breast Cancer Stem Cells

Published on: January 18, 2017

实现脂聚糖诱导NF-kappaB激活的稳定性

Markus W Covert1, Thomas H Leung, Jahlionais E Gaston

  • 1Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA.

Science (New York, N.Y.)
|September 17, 2005
PubMed
概括

瘤坏死因子-α (TNFalpha) 刺激会导致NF-kappaB激活的减弱振荡,与脂聚糖 (LPS) 的稳定行为不同. 一个计算模型显示,一个途径的时间延迟对于NF-kappaB激活动态至关重要.

科学领域:

  • 蜂信号传输是如何进行的
  • 免疫学 免疫学 免疫学
  • 系统生物学 系统生物学

背景情况:

  • 核因子-卡帕B (NF-kappaB) 是免疫反应中的关键转录因子.
  • 根据刺激,NF-kappaB的激活动态有所不同,例如瘤亡因子-α (TNFalpha) 和脂多糖 (LPS).
  • 收费类受体4 (TLR4) 通过不同的下游信号通路调解LPS诱导的NF-kappaB激活.

研究的目的:

  • 研究NF-kappaB.的差异激活动态背后的机制.
  • 阐明信号通路定时在NF-kappaB调控中的作用.
  • 以计算方式建模TLR4依赖的NF-kappaB信号网络.

主要方法:

  • 用TNFalpha和LPS刺激细胞.
  • 对NF-kappaB激活动态的分析.
  • 开发和应用用于信号通路的计算模型.
  • 研究个别途径对整体NF-kappaB行为的贡献.

主要成果:

  • 在TNFalpha刺激时,NF-kappaB表现出抑制的振荡行为,但在LPS刺激时表现出稳定的动态.
  • 在LPS诱导的NF-kappaB激活中,有两个不同的TLR4-依赖的通路参与其中.

更多相关视频

A Macrophage Reporter Cell Assay to Examine Toll-Like Receptor-Mediated NF-kB/AP-1 Signaling on Adsorbed Protein Layers on Polymeric Surfaces
07:55

A Macrophage Reporter Cell Assay to Examine Toll-Like Receptor-Mediated NF-kB/AP-1 Signaling on Adsorbed Protein Layers on Polymeric Surfaces

Published on: January 7, 2020

NF-κB-dependent Luciferase Activation and Quantification of Gene Expression in Salmonella Infected Tissue Culture Cells
10:57

NF-κB-dependent Luciferase Activation and Quantification of Gene Expression in Salmonella Infected Tissue Culture Cells

Published on: January 12, 2020

相关实验视频

Last Updated: Jul 16, 2026

Synthesis and Characterization of an Aspirin-fumarate Prodrug that Inhibits NFκB Activity and Breast Cancer Stem Cells
13:38

Synthesis and Characterization of an Aspirin-fumarate Prodrug that Inhibits NFκB Activity and Breast Cancer Stem Cells

Published on: January 18, 2017

A Macrophage Reporter Cell Assay to Examine Toll-Like Receptor-Mediated NF-kB/AP-1 Signaling on Adsorbed Protein Layers on Polymeric Surfaces
07:55

A Macrophage Reporter Cell Assay to Examine Toll-Like Receptor-Mediated NF-kB/AP-1 Signaling on Adsorbed Protein Layers on Polymeric Surfaces

Published on: January 7, 2020

NF-κB-dependent Luciferase Activation and Quantification of Gene Expression in Salmonella Infected Tissue Culture Cells
10:57

NF-κB-dependent Luciferase Activation and Quantification of Gene Expression in Salmonella Infected Tissue Culture Cells

Published on: January 12, 2020

  • 计算建模表明,一个途径的时间延迟对于通过两个途径实现NF-kappaB的反相激活至关重要.
  • MyD88独立途径的延迟是由TNFalpha合成所需的时间建立的.

    • 结论:

    • 不同的信号通路的时间和相互作用对于确定NF-kappaB激活动态至关重要.
    • 时间延迟,特别是在涉及TNFalpha合成的MyD88独立途径中,是关键的调节机制.
    • 了解这些动态,可以了解免疫细胞的反应和潜在的治疗点.