相关实验视频

Updated: Jul 8, 2026

Genome-wide Protein-protein Interaction Screening by Protein-fragment Complementation Assay (PCA) in Living Cells
08:38

Genome-wide Protein-protein Interaction Screening by Protein-fragment Complementation Assay (PCA) in Living Cells

Published on: March 3, 2015

一个人类蛋白质-蛋白质相互作用网络:用于注释蛋白质组的资源.

Ulrich Stelzl1, Uwe Worm, Maciej Lalowski

  • 1Max Delbrueck Center for Molecular Medicine, 13092 Berlin-Buch, Germany.

Cell
|September 20, 2005
PubMed
概括

这项研究使用酵母二混合查系统地绘制了人类蛋白质与蛋白质相互作用的地图,揭示了3186个相互作用的庞大网络. 确定了高可信度相互作用,揭示了对细胞通路和蛋白质功能的新见解.

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Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a polypeptide...
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These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...