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相关概念视频

Phosphodiester Linkages01:01

Phosphodiester Linkages

Overview
Phosphodiester bond forms when a phosphoric acid molecule (H3PO4) links with two hydroxyl groups (–OH) of two other molecules, forming two ester bonds. Two water molecules are released in this process. The phosphodiester bond is commonly found in nucleic acids (DNA and RNA) and plays a critical role in their structure and function.
Phosphodiester Bonds Link Nucleotides Together
DNA and RNA are polynucleotides or long chains of nucleotides that are linked together. A nucleotide is...
RNA Structure01:23

RNA Structure

Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
Eukaryotic RNA Polymerases00:58

Eukaryotic RNA Polymerases

RNA Polymerase (RNAP) is conserved in all animals, with bacterial, archaeal, and eukaryotic RNAPs sharing significant sequence, structural, and functional similarities. Among the three eukaryotic RNAPs, RNA Polymerase II is most similar to bacterial RNAP in terms of both structural organization and folding topologies of the enzyme subunits. However, these similarities are not reflected in their mechanism of action.
All three eukaryotic RNAPs require specific transcription factors, of which the...
Ribosomal RNA Synthesis02:53

Ribosomal RNA Synthesis

Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
RNA Structure01:19

RNA Structure

The basic structure of RNA consists of a string of ribonucleotides attached by phosphodiester bonds. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...
Eukaryotic RNA Polymerases00:58

Eukaryotic RNA Polymerases

RNA Polymerase (RNAP) is conserved in all animals, with bacterial, archaeal, and eukaryotic RNAPs sharing significant sequence, structural, and functional similarities. Among the three eukaryotic RNAPs, RNA Polymerase II is most similar to bacterial RNAP in terms of both structural organization and folding topologies of the enzyme subunits. However, these similarities are not reflected in their mechanism of action.
All three eukaryotic RNAPs require specific transcription factors, of which the...

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相关实验视频

Updated: Jul 10, 2026

Highly Efficient Ligation of Small RNA Molecules for MicroRNA Quantitation by High-Throughput Sequencing
14:15

Highly Efficient Ligation of Small RNA Molecules for MicroRNA Quantitation by High-Throughput Sequencing

Published on: November 18, 2014

一般去氧化酶催化合成原生3'-5'RNA链接的合成.

Whitney E Purtha1, Rebecca L Coppins, Mary K Smalley

  • 1Department of Chemistry, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, USA.

Journal of the American Chemical Society
|September 22, 2005
PubMed
概括

研究人员开发了用于RNA结合的新型脱氧酶,它们快速,序列通用,并产生高产量的本地链接. 这些新型脱氧化酶 (9DB1和7DE5) 在分子生物学和生物技术中提供了实际应用.

科学领域:

  • 分子生物学分子生物学
  • 生物化学 生物化学
  • 酶学 是一种酶学.

背景情况:

  • 在核酸酶学中,开发具有高效率和序列普遍性的脱氧酶用于RNA结合仍然是一个重大挑战.
  • 现有的脱氧酶通常在速度,序列兼容性,链接忠实性或产量方面存在局限性,这阻碍了它们的广泛应用.

研究的目的:

  • 识别能够快速,序列通用RNA结合与本地3'-5'链接的新型脱氧酶,并准备有用的产量.
  • 描述用于RNA结合的新发现的脱氧核糖酶的催化性质和基质序列要求.

主要方法:

  • 利用体外选择来发现和分离具有所需RNA结合能力的脱氧酶.
  • 在实践化条件下评估已识别的脱氧酶的性能,评估结合率,序列普遍性,链接忠实性和产量.
  • 确定了新型脱氧核糖酶的特定RNA基质序列要求.

主要成果:

  • 成功确定了依赖Mg2+和Zn2+的脱氧酶 (9DB1和7DE5),这些脱氧酶满足了有效RNA结合的所有标准.
  • 这些脱氧酶在实际条件下运作,并表现出适度的序列要求 (D↓RA为9DB1,A↓R为7DE5),与已建立的RNA分裂脱氧酶相比.
  • 在准备有用的产量中证明了原生3'-5'二键的形成.

更多相关视频

2D-HELS MS Seq: A General LC-MS-Based Method for Direct and de novo Sequencing of RNA Mixtures with Different Nucleotide Modifications
05:41

2D-HELS MS Seq: A General LC-MS-Based Method for Direct and de novo Sequencing of RNA Mixtures with Different Nucleotide Modifications

Published on: July 10, 2020

Chemical Triphosphorylation of Oligonucleotides
13:19

Chemical Triphosphorylation of Oligonucleotides

Published on: June 2, 2022

相关实验视频

Last Updated: Jul 10, 2026

Highly Efficient Ligation of Small RNA Molecules for MicroRNA Quantitation by High-Throughput Sequencing
14:15

Highly Efficient Ligation of Small RNA Molecules for MicroRNA Quantitation by High-Throughput Sequencing

Published on: November 18, 2014

2D-HELS MS Seq: A General LC-MS-Based Method for Direct and de novo Sequencing of RNA Mixtures with Different Nucleotide Modifications
05:41

2D-HELS MS Seq: A General LC-MS-Based Method for Direct and de novo Sequencing of RNA Mixtures with Different Nucleotide Modifications

Published on: July 10, 2020

Chemical Triphosphorylation of Oligonucleotides
13:19

Chemical Triphosphorylation of Oligonucleotides

Published on: June 2, 2022

结论:

  • 新发现的9DB1和7DE5脱氧化酶代表了RNA结合技术的重大进步.
  • 这些酶由于其效率和实用性,预计将成为分子生物学,合成生物学和生物技术中各种应用的有价值工具.
  • 这些发现为开发用于复杂RNA操纵的催化核酸开辟了新的途径.