德洛索菲拉翅膀形象性磁盘细胞因子对Decapentaplegic的依赖性
Frank Hsiung1, Felipe-Andrès Ramirez-Weber, D David Iwaki
1Department of Biochemistry and Biophysics, University of California, San Francisco, California 94143, USA.
Nature
|September 24, 2005
概括
细胞突出称为细胞膜,有助于分发发育信号,如在Drosophila翅膀盘中的Decapentaplegic (Dpp) 和Wingless (Wg). 这些发现表明,细胞膜在细胞间通信和发育模式中起着至关重要的作用.
科学领域:
- 发展生物学 发展生物学
- 细胞生物学 细胞生物学
- 遗传学 是一个遗传学.
背景情况:
- 在Drosophila中,翅膀影像盘是由前后和背后/腹腔区边界的组织中心构成的.
- 十足 (Dpp) 和无翼 (Wg) 是由这些组织者产生的关键信号分子,调节生长和模式.
- Dpp和Wg分布的确切机制尚不清楚,提出的模型包括扩散和囊泡传输.
研究的目的:
- 研究细胞膜在Dpp和Wg信号分子分布中的作用.
- 为了确定细胞膜是否参与Drosophila翅膀盘中生长和模式的非自主调节.
主要方法:
- 与A/P和D/V组织者相比,在Drosophila翅膀盘中观察细胞膜方向.
- 细胞膜存在/定向与DPP信号之间的相关性分析.
- 在细胞膜上定位和跟踪DPP受体,Thickveins (Tkv).
主要成果:
- 观察到翅膀盘中的细胞膜都朝着A/P和D/V组织者方向.
- 细胞因子的存在和方向与DPP信号正相关.
- 发现DPP受体 (Tkv) 在细胞膜沿移动点体中.
结论:
- 细胞膜参与了Drosophila翅膀盘中信号分子的定向运输.
- 这些发现支持一种模型,其中细胞膜调解细胞间通信,用于发育模式.
- 细胞因子可以通过受体运动作为Dpp信号转导的导管.
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