西尼罗河病毒通过治疗性抗体中和的结构基础
Grant E Nybakken1, Theodore Oliphant, Syd Johnson
1Department of Pathology, Washington University School of Medicine, St Louis, Missouri 63110, USA.
Nature
|September 30, 2005
概括
西尼罗病毒被E16抗体中和的目标域是III (DIII) 域,这是一个关键的弗拉维病毒表位. 这种抗体在附着后阻断病毒感染,这表明了针对黄病毒病的潜在疫苗策略.
科学领域:
- 病毒学 病毒学
- 免疫学 免疫学 免疫学
- 结构生物学 结构生物学
背景情况:
- 西尼罗河病毒 (WNV) 是一种由蚊子传播的病毒,与严重的人类疾病有关.
- 弗拉维病毒感染涉及内体膜融合,由信封糖蛋白构造变化驱动.
- 幽默免疫对于早期预防WNV感染至关重要.
研究的目的:
- 为了研究E16单克隆抗体对西尼罗河病毒的中和机制.
- 在WNV包裹上以E16识别的表位表征糖蛋白域III (DIII).
- 探索向DIII表位的潜力,以开发flaviviral疾病疫苗.
主要方法:
- 对与WNV DIII结合的E16抗体Fab片段的结构分析.
- 在整个病毒中识别DIII上保存的中和表位.
- 在接研究中预测抗体-表皮质相互作用.
- 抑制试验用于确定被E16阻断的病毒感染阶段.
主要成果:
- E16抗体与四个DIII环上的16个残留物结合,形成一个保存的中和表位.
- 这种表位被暴露在维表面上,其与E16的结合可能会暴露其他表位.
- E16在病毒附着后抑制了WNV感染,可能是通过防止必要的糖蛋白构造变化来阻止.
- 已识别的表位在WNV中保存,但在其他黄病毒中是不同的.
结论:
- E16抗体通过向域III上的特定表位体来中和西尼罗病毒.
- 准这种保存的DIII表位可能会提供针对黄病毒性疾病的广泛疫苗策略.
- E16的机制涉及阻断附着后的病毒进入步骤,突出显示潜在的治疗点.
相关概念视频
01:10Antibody Structure
Overview
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
00:42Cross-reactivity
Overview
01:28Rabies
Rabies is a lethal zoonotic disease caused by a single-stranded, negative-sense RNA virus of the Lyssavirus genus, within the family Rhabdoviridae. Its primary mode of transmission to humans is through bites or saliva-contaminated scratches from infected mammals such as dogs, bats, raccoons, or foxes. Transmission can also occur if infectious saliva contacts abraded skin or intact mucous membranes, including the conjunctiva.Viral Entry and Early ReplicationOnce introduced at the bite or scratch...