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相关概念视频

Recombinant DNA01:09

Recombinant DNA

Overview
DNA as a Genetic Template02:05

DNA as a Genetic Template

Two structural features of the DNA molecule provide a basis for the mechanisms of heredity: the four nucleotide bases and its double-stranded nature. The Watson-Crick model of double-helical DNA structure, proposed in 1952, drew heavily upon the X-ray crystallography work of researchers Rosalind Franklin and Maurice Wilkins. Watson, Crick, and Wilkins jointly received the Nobel Prize in Physiology or Medicine for their work in 1962. Franklin was, controversially, excluded from the prize for...
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
Recombinant DNA01:09

Recombinant DNA

Overview
DNA as a Genetic Template02:05

DNA as a Genetic Template

Two structural features of the DNA molecule provide a basis for the mechanisms of heredity: the four nucleotide bases and its double-stranded nature. The Watson-Crick model of double-helical DNA structure, proposed in 1952, drew heavily upon the X-ray crystallography work of researchers Rosalind Franklin and Maurice Wilkins. Watson, Crick, and Wilkins jointly received the Nobel Prize in Physiology or Medicine for their work in 1962. Franklin was, controversially, excluded from the prize for...
DNA Bacteriophages01:26

DNA Bacteriophages

Bacteriophages, or phages, are viruses that specifically infect bacteria, utilizing their genetic material to hijack host cellular machinery for replication. DNA bacteriophages employ single-stranded DNA (ssDNA) or double-stranded DNA (dsDNA) genomes. These phages exhibit diverse replication strategies and host interactions, influencing their ecological roles and applications in biotechnology and medicine.ssDNA BacteriophagesssDNA phages, with their small genomes, utilize unique strategies to...

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相关实验视频

Updated: Jul 15, 2026

A Simple and Efficient Approach to Construct Mutant Vaccinia Virus Vectors
09:16

A Simple and Efficient Approach to Construct Mutant Vaccinia Virus Vectors

Published on: October 30, 2016

Rev1采用一种使用蛋白质模板合成DNA的新机制.

Deepak T Nair1, Robert E Johnson, Louise Prakash

  • 1Structural Biology Program, Department of Physiology and Biophysics, Mount Sinai School of Medicine, Box 1677, 1425 Madison Avenue, New York, NY 10029, USA.

Science (New York, N.Y.)
|October 1, 2005
PubMed
概括

Rev1 DNA聚合酶独特地结合了与模板G相对的核酸.它通过驱逐模板G并将传入的核酸与氨酸残留物配对来实现这一目标,确保精确的DNA合成.

科学领域:

  • 生物化学 生物化学
  • 分子生物学分子生物学
  • 结构生物学 结构生物学

背景情况:

  • Rev1 DNA聚合酶在DNA修复和复制中起着至关重要的作用.
  • 它的特点是它能够将细胞因子与模板关氨基基相对应的能力而闻名.
  • 了解Rev1的机制对于理解细胞对DNA损伤的反应至关重要.

研究的目的:

  • 阐明Rev1的专门核酸合并机制的结构基础.
  • 为了研究Rev1如何区分模板和输入核酸.
  • 提供对无错误DNA合成的洞察力,特别是在受损的瓜宁部位.

主要方法:

  • 使用X射线晶体学来确定酵母Rev1.的结构.
  • 晶体结构捕获了Rev1与模板关氨酸和输入的2'-脱氧化丁5'-三酸盐 (dCTP) 的复合体.

主要成果:

  • 晶体结构显示Rev1决定了模板基和输入核酸的身份.
  • 模板关氨酸从DNA螺旋体中被驱逐出来,并与Rev1形成键.
  • 传入的dCTP与聚合酶活性部位内的氨酸残留物配对,而不是模板基.

结论:

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Protocols for C-Brick DNA Standard Assembly Using Cpf1
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Protocols for C-Brick DNA Standard Assembly Using Cpf1

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A Seamless Cloning Approach for Porcine Reproductive and Respiratory Syndrome Virus Expression Vector Construction
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A Seamless Cloning Approach for Porcine Reproductive and Respiratory Syndrome Virus Expression Vector Construction

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相关实验视频

Last Updated: Jul 15, 2026

A Simple and Efficient Approach to Construct Mutant Vaccinia Virus Vectors
09:16

A Simple and Efficient Approach to Construct Mutant Vaccinia Virus Vectors

Published on: October 30, 2016

Protocols for C-Brick DNA Standard Assembly Using Cpf1
12:03

Protocols for C-Brick DNA Standard Assembly Using Cpf1

Published on: June 15, 2017

A Seamless Cloning Approach for Porcine Reproductive and Respiratory Syndrome Virus Expression Vector Construction
04:18

A Seamless Cloning Approach for Porcine Reproductive and Respiratory Syndrome Virus Expression Vector Construction

Published on: May 17, 2024

  • Rev1采用一种独特的机制,与其他DNA聚合酶不同,以确保dCTP的纳入.
  • 这种机制涉及模板基驱逐和与氨酸残留物相互作用.
  • 这一过程促进了熟练和无错误的DNA合成,特别是当遇到阻碍复制的N2吸附关氨酸时.