哈尔,引起植物病原体Erwinia amylovora产生的过敏反应的诱因
Z M Wei1, R J Laby, C H Zumoff
1Department of Plant Pathology, Cornell University, Ithaca, NY 14853.
概括
科学家分离了harpin,一种来自Erwinia amylovora的蛋白质,它触发了植物防御反应,如过敏反应. 哈尔 哈尔 哈尔 哈尔 哈尔
科学领域:
- 植物病理学 植物病理学
- 微生物遗传学微生物遗传学
- 分子生物学分子生物学
背景情况:
- 埃尔温亚amyylovora在粉红色植物中引起火灾.
- 过敏反应是一种植物的防御机制.
- 这种反应的蛋白质诱导者以前没有被表征.
研究的目的:
- 为了隔离和表征由Erwinia amylovora引起的过敏反应的蛋白质诱因.
- 为了识别和分析编码这种诱导者的基因.
- 调查这种诱导体在病原性和植物防御中的作用.
主要方法:
- 蛋白质诱导物的分离和净化.
- 生物化学特征 (pH,热稳定性,分子量).
- 基因克隆,测序和突变发生 (Tn5tac1).
- 对梨的致病性测定.
- 对烟草的过敏反应测定.
主要成果:
- 一种叫做harpin的蛋白质性诱导物被分离出来.
- 哈尔酸性,热稳定,44kDa,与细胞外相关.
- 哈尔诱导了叶片膜的崩,并增加了烟草细胞中的外部pH值.
- 哈尔基因 (hrpN) 在hrp基因集群中被确定.
- hrpN突变是非致病的,不引起过敏反应,缺乏哈尔的生产.
结论:
- 哈尔是一种Erwinia amylovora的关键毒性因子.
- 哈尔直接引起了植物的过敏反应.
- hrpN基因对于哈尔的产生,致病性和过敏反应的产生至关重要.
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