多基化因子CPSF-73参与了基因素-前mRNA处理
Zbigniew Dominski1, Xiao-cui Yang, William F Marzluff
1Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill, NC 27599, USA. dominski@med.unc.edu
Cell
|October 11, 2005
概括
基因组前mRNA处理涉及由U7小核核核糖核蛋白 (snRNP) 依赖核酶的分裂和降解. 研究人员确定CPSF-73是关键蛋白质,在这个关键的RNA处理途径中负责内核酶和外核酶活动.
科学领域:
- 分子生物学分子生物学
- 处理RNA处理RNA处理
- 基因表达 基因表达
背景情况:
- 基因组前mRNA经历3'端处理,包括裂变和降解.
- 这个过程依赖于U7小核核糖核蛋白 (snRNP).
- 涉及两个不同的核酶活动:一个内核酶和一个5'-3'外核酶.
研究的目的:
- 为了识别在3'末端处理中负责内核酶和外核酶活动的特定蛋白质.
- 阐明U7 snRNP依赖核酶的作用.
主要方法:
- 使用改性RNA基质进行了UV交叉链接研究.
- 基质包括完整的RNA和下游裂变产品.
- 免疫沉被用来识别交联蛋白质.
主要成果:
- 一种以85kDa的速度迁移的蛋白质被检测到以U7依赖的方式与两个RNA基质交叉连接.
- 免疫沉识别了这个85kDa的蛋白质为CPSF-73.
- CPSF-73参与了裂变和降解步骤.
结论:
- 在基因组前mRNA处理中,CPSF-73既可以作为内核酶,也可以作为5'-3'外核酶.
- 这一发现揭示了基因组 mRNA 3' 末端形成和多基化 mRNA 处理之间的进化联系.
相关概念视频
02:01pre-mRNA Processing
In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl guanosine). This 5’ cap helps the...
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl guanosine). This 5’ cap helps the...
02:01Pre-mRNA Processing
In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl guanosine). This 5’ cap helps the...
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl guanosine). This 5’ cap helps the...
02:41Chromatin Structure Regulates pre-mRNA Processing
In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
The chromatin structure, especially...
02:51mRNA Stability and Gene Expression
The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
Cis-acting Elements involved in mRNA stability
01:35Pre-mRNA Processing: Modification of pre-mRNA Ends
In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a cap to the 5' end of the growing transcript. In this process, a 5' phosphate is replaced by modified guanosine that has a methyl group attached (7-methyl guanosine). This 5' cap helps the cell...
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a cap to the 5' end of the growing transcript. In this process, a 5' phosphate is replaced by modified guanosine that has a methyl group attached (7-methyl guanosine). This 5' cap helps the cell...
02:51mRNA Stability and Gene Expression
The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
Cis-acting Elements involved in mRNA stability