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水性溶剂与玻璃型溶剂对蛋白质动态的影响:振动回声实验和分子动力学模拟

Aaron M Massari1, Ilya J Finkelstein, Brian L McClain

  • 1Department of Chemistry, Stanford University, Stanford, CA 94305, USA.

Journal of the American Chemical Society
|October 13, 2005
PubMed
概括

与水溶液相比,Trehalose玻璃显著减缓蛋白质动力学和光谱扩散,通过减轻原子位移. 这种生物保护机制涉及通过受约束的水层将蛋白质表面固定起来.

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Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...