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Cholesterol Efflux Assay
Published on: March 6, 2012
血清降低细胞乙CoA:胆固醇乙转移酶活性的能力预测心血管结果
Julio A Chirinos1, Juan P Zambrano, Simon Chakko
1Miller School of Medicine, University of Miami, Miami, FL 33138, USA.
Circulation
|October 19, 2005
概括
血清胆固醇外流活动,以其降低胆固醇以使胆固醇变质的能力来衡量,独立预测男性的主要不良心血管事件 (MACE) 和死亡. 这一发现为评估心血管风险提供了新的见解.
科学领域:
- 心血管医学 心血管医学
- 生物化学 生物化学
- 脂质代谢 脂质代谢是什么
背景情况:
- 研究了血清胆固醇外流活动与冠状动脉疾病 (CAD) 之间的关联.
- 研究了胆固醇排放与重大心血管不良事件 (MACE) 和死亡风险之间的联系.
研究的目的:
- 为了确定血清胆固醇排泄活动是否预测血管学CAD的存在.
- 评估胆固醇外流活动与MACE和死亡风险的关联.
主要方法:
- 研究了168名接受冠状动脉血管造影的男性.
- 使用患者血清和人类皮肤纤维细胞测量了体外胆固醇排泄活性.
- 通过血清降低可用于acylCoA:胆固醇乙烯转移酶 (ACAT) 化可用的胆固醇的能力来定义排泄活性.
- 随访患者4.5年来跟踪MACE和死亡.
主要成果:
- 血清诱导的ACAT活性变化与HDL水平或CAD存在没有相关性.
- 在ACAT活性变化的最高三位数中的患者,MACE (HR,2.15) 和死亡 (HR,2.23) 的风险明显更高.
- 这些相关性与其他风险标志物 (如LDL,HDL和C反应蛋白) 保持独立.
结论:
- 血清能够耗尽细胞胆固醇以进行ACAT化,这是MACE和死亡的强大,独立的预测因子.
- 据推测,这种活动涉及ATP结合盒载体A1 (ABCA1) 介导的流动.
- 假设血清中影响ACAT的HDL颗粒增加与体内有限的ABCA1活性有关.
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