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相关概念视频

Contact-dependent Signaling01:19

Contact-dependent Signaling

Contact-dependent signaling, as the name suggests, requires that communicating cells be in direct contact with each other. This is achieved either through receptor-ligand interactions or by specialized cytoplasmic channels that allow the flow of small molecules between cells. In animal cells, channels called gap junctions facilitate contact-dependent signaling in certain tissues, whereas, plasmodesmata perform a similar function in plants.
Gap Junctions
In animal cells, gap junctions are formed...
Defenses Against Pathogens and Herbivores02:26

Defenses Against Pathogens and Herbivores

Plants present a rich source of nutrients for many organisms, making it a target for herbivores and infectious agents. Plants, though lacking a proper immune system, have developed an array of constitutive and inducible defenses to fend off these attacks.
Protein Transport to the Outer Chloroplast Membrane01:11

Protein Transport to the Outer Chloroplast Membrane

Chloroplast outer membrane proteins encoded by the nucleus are synthesized in the cytosol. Soon after synthesis, they bind cytosolic factors such as 14-3-3 protein and the Hsp70 chaperones that keep these precursors in an unfolded state until their translocation.
Two models describe the mechanism of precursor recognition and entry across the outer membrane through the TOC complex. Model 1 suggests the newly synthesized precursor binds to the TOC receptor 159 and forms a complex.
Cell Motility through Blebbing01:16

Cell Motility through Blebbing

Blebs are a type of membrane protrusion formed by the internal hydrostatic pressure of the cytoplasm. Blebs are observed in several cell types, including fibroblasts, immune cells, and single-celled organisms like the amoeba. The primary function of blebs is cell locomotion and apoptosis, but they are also found during necrosis and cell division. The life cycle of a bleb comprises an initiation phase followed by the expansion and retraction phases.
Blebbing Through the Matrix
In multicellular...
Types of Membrane Protrusions01:28

Types of Membrane Protrusions

The protrusion of the cell surface is an initial step for several cellular processes, including cell migration, phagocytosis, and neurite outgrowth. These membrane protrusions are a result of cytoskeletal rearrangement. The most  widely observed cell protrusions include lamellipodia, pseudopodia, filopodia, microvilli, invadopodia, and podosomes. These protrusions can be of two types — static or dynamic.
The microvilli, an example of stable protrusions, are finger-like projections with a...
Cell Adhesion in Plants01:14

Cell Adhesion in Plants

Plants have rigid cell walls that are made up of cell wall polysaccharides that mediate cell-cell adhesion. The primary cell walls of plants consist of two independent and interacting polysaccharide networks: a pectin matrix that embeds the second network comprising cellulose and hemicelluloses.
Pectins are complex heteropolymers mainly composed of negatively-charged α-D-glucopyranosyl uronic acid and some neutral glycosyl residues such as α-L-rhamnopyranose, α-L-arabinofuranose, and...

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相关实验视频

Updated: Jul 12, 2026

Live Imaging Assay for Assessing the Roles of Ca2+ and Sphingomyelinase in the Repair of Pore-forming Toxin Wounds
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Live Imaging Assay for Assessing the Roles of Ca2+ and Sphingomyelinase in the Repair of Pore-forming Toxin Wounds

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一种富含proline的植物细胞壁蛋白的诱导和伤口诱导的氧化交叉链接:一种新的,快速的防御反应.

D J Bradley1, P Kjellbom, C J Lamb

  • 1Plant Biology Laboratory, Salk Institute for Biological Studies, La Jolla, California 92037.

Cell
|July 10, 1992
PubMed
概括

诱导剂触发植物细胞壁蛋白的快速交叉链接,这是防御基因激活之前的过程. 这种氧化交叉连接在生长和压力期间加强细胞壁.

科学领域:

  • 植物生物学 植物生物学
  • 分子生物学分子生物学
  • 生物化学 生物化学

背景情况:

  • 植物细胞壁含有 () 丰富的结构蛋白质.
  • 这些蛋白质在细胞壁完整性和植物防御方面发挥作用.
  • 细胞对外部刺激的反应对于植物的生存至关重要.

研究的目的:

  • 为了研究在用真菌诱导剂或谷氨治疗时细胞壁蛋白的快速变化.
  • 阐明植物细胞壁中蛋白质不溶化的机制和时间.
  • 了解氧化交叉连接在植物防御和发育中的作用.

主要方法:

  • 用真菌诱导剂和谷氨治疗豆和大豆细胞.
  • 在细胞壁中分析富含 () 的结构蛋白.
  • 研究过氧化 (H2O2) 介导的氧化交叉链接.
  • 蛋白质不溶化的时间和与防御基因表达的比较.

主要成果:

  • 菌诱导剂或谷氨治疗迅速使细胞壁中先前存在的 (氧) 蛋白丰富的结构蛋白质难以溶解.
  • 不溶性通过H2O2介导的氧化交叉连接发生,在2分钟内启动,并在10分钟内完成.
  • 这种快速的交叉链接先于转录依赖防御的表达.

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Pattern-Triggered Oxidative Burst and Seedling Growth Inhibition Assays in Arabidopsis thaliana

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Live Imaging Assay for Assessing the Roles of Ca2+ and Sphingomyelinase in the Repair of Pore-forming Toxin Wounds
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Live Imaging Assay for Assessing the Roles of Ca2+ and Sphingomyelinase in the Repair of Pore-forming Toxin Wounds

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High-throughput Measurement of Plasma Membrane Resealing Efficiency in Mammalian Cells

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Pattern-Triggered Oxidative Burst and Seedling Growth Inhibition Assays in Arabidopsis thaliana
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Pattern-Triggered Oxidative Burst and Seedling Growth Inhibition Assays in Arabidopsis thaliana

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  • 氧化交叉连接也在发育上受到调节,并且在机械应力下发生.
  • 结论:

    • 细胞壁蛋白的刺激依赖氧化交叉链接是植物中一种新的调节机制.
    • 这个过程是植物防御的早期事件,发生在转录变化之前.
    • 氧化交叉连接在应激和发育期间有助于细胞成熟和细胞壁强化.