光系统II核心酸化和光合作用适应需要两个不同的蛋白激酶
Vera Bonardi1, Paolo Pesaresi, Thomas Becker
1Botanisches Institut, Department Biologie I, Ludwig-Maximilians-Universität, Menzinger Strasse 67, 80638 München, Germany.
Nature
|October 21, 2005
概括
光合作用光适应涉及蛋白质酸化. STN7激酶调节状态转换,而STN8激酶化光系统II核心蛋白质,影响基因表达和能量分配.
科学领域:
- 植物生物学 植物生物学
- 光合作用研究研究光合作用.
- 分子植物生理学分子植物生理学
背景情况:
- 照明的变化引发了甲状腺蛋白的修饰和光合作用机制的重组.
- 短期适应包括光系统II (PSII) 和光收获蛋白质 (LHCII) 的酸化,影响PSII周转和激发能量再分配 (状态转换).
- 长期的适应包括调整光系统固态度以平衡能量分布.
研究的目的:
- 调查STN7和STN8蛋白激酶在Arabidopsis中光合作用适应中的作用.
- 确定STN8对PSII核心蛋白质酸化的必要性,用于PSII修复和适应.
- 为了阐明短期和长期光合作用适应之间的合.
主要方法:
- 对缺乏STN7和STN8激酶的阿拉比多普西斯突变物进行分析.
- 在高强度光线下评估PSII活动和D1蛋白循环.
- 评估适应光质变化的适应情况.
主要成果:
- STN8对于PSII核心蛋白质的定量化至关重要,但它缺少对PSII活动和在高光下D1周转有很小的影响.
- 需要STN7,而不是STN8来适应光质的变化.
- 在stn7突变体中适应的缺陷表明短期和长期光合作用适应之间的合.
结论:
- 可逆的蛋白质酸化对于PSII修复并非必不可少.
- STN7激酶在状态转换和光合作用基因表达的控制中起着至关重要的作用,将短期和长期适应联系起来.
- 化LHCII或未知的STN7基质对于调节光合作用基因表达至关重要.
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