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相关概念视频

DNA Packaging00:58

DNA Packaging

Overview
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
Recombinant DNA01:09

Recombinant DNA

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DNA Isolation01:24

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
DNA Bacteriophages01:26

DNA Bacteriophages

Bacteriophages, or phages, are viruses that specifically infect bacteria, utilizing their genetic material to hijack host cellular machinery for replication. DNA bacteriophages employ single-stranded DNA (ssDNA) or double-stranded DNA (dsDNA) genomes. These phages exhibit diverse replication strategies and host interactions, influencing their ecological roles and applications in biotechnology and medicine.ssDNA BacteriophagesssDNA phages, with their small genomes, utilize unique strategies to...

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相关实验视频

Updated: Jul 1, 2026

Automated Robotic Liquid Handling Assembly of Modular DNA Devices
11:22

Automated Robotic Liquid Handling Assembly of Modular DNA Devices

Published on: December 1, 2017

在高密度下实现可变DNA功能化的多功能工具箱.

Stefan Jäger1, Goran Rasched, Hagit Kornreich-Leshem

  • 1Kekuké-Institut für Organische Chemie und Biochemie, Universität Bonn, Gerhard-Domagk-Strasse 1, D-53121 Bonn, Germany.

Journal of the American Chemical Society
|October 27, 2005
PubMed
概括
此摘要是机器生成的。

化学修饰DNA (fDNA) 可以通过使用原料延伸和PCR与修饰核酸合成. 酶选择和序列组成影响fDNA合成,对DNA结构和应用有影响.

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Design and Synthesis of a Reconfigurable DNA Accordion Rack
07:44

Design and Synthesis of a Reconfigurable DNA Accordion Rack

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CRISPR-Based Modular Assembly for High-Throughput Construction of a UAS-cDNA/ORF Plasmid Library
05:30

CRISPR-Based Modular Assembly for High-Throughput Construction of a UAS-cDNA/ORF Plasmid Library

Published on: May 17, 2024

相关实验视频

Last Updated: Jul 1, 2026

Automated Robotic Liquid Handling Assembly of Modular DNA Devices
11:22

Automated Robotic Liquid Handling Assembly of Modular DNA Devices

Published on: December 1, 2017

Design and Synthesis of a Reconfigurable DNA Accordion Rack
07:44

Design and Synthesis of a Reconfigurable DNA Accordion Rack

Published on: August 15, 2018

CRISPR-Based Modular Assembly for High-Throughput Construction of a UAS-cDNA/ORF Plasmid Library
05:30

CRISPR-Based Modular Assembly for High-Throughput Construction of a UAS-cDNA/ORF Plasmid Library

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科学领域:

  • 化学生物学 化学生物学
  • 分子生物学分子生物学
  • 生物技术是生物技术.

背景情况:

  • 化学改性DNA (fDNA) 对于纳米技术,材料科学和传感器开发的应用至关重要.
  • 目前的方法需要策略,同时引入多种修改到单个DNA序列.

研究的目的:

  • 通过原始扩展和PCR来研究合成高密度功能化DNA (fDNA) 的可行性和局限性.
  • 探索改性脱氧核酸三酸盐 (dNTP),DNA聚合酶和模板对fDNA合成的影响.

主要方法:

  • 使用各种化学修饰的dNTP,DNA聚合酶和模板的原料扩展和PCR.
  • 用多达四种不同的基基修饰的类似物代替天然核基.
  • 使用CD光谱学分析了酶放大和形状变化的序列依赖性.

主要成果:

  • 酶放大效率取决于序列组成 (AT丰富者优先于GC丰富者) 和DNA聚合酶家族 (B家族优于A家族对于具有挑战性的模板).
  • 单基基改造不会改变B型DNA结构.
  • 三个修改基的密度会诱导形状变化,可作为CD光谱反转观察.

结论:

  • 为生成高密度fDNA的多功能工具箱奠定了基础.
  • 证明了序列和聚合酶选择对fDNA合成的影响.
  • 突出了高密度DNA修饰的结构后果.