通过Dishevelled进行致命的巨型幼虫的调控
Gretchen L Dollar1, Ursula Weber, Marek Mlodzik
1Department of Molecular, Cell and Developmental Biology, Box 1020, Mount Sinai School of Medicine, One Gustave L. Levy Place, New York, New York 10029, USA.
Nature
|October 28, 2005
概括
致命的巨型幼虫 (Lgl) 蛋白质的定位和活性,对于细胞极性至关重要,由Dishevelled (Dsh) 和Wnt信号调节. 这揭示了在发育过程中控制细胞两极分化的分子机制.
科学领域:
- 细胞生物学 细胞生物学
- 发育生物学是发展生物学.
- 分子信号传递是分子信号传递.
背景情况:
- 细胞极性对于组织发育和功能至关重要.
- 致命巨 (Lgl) 蛋白质是细胞极性和底侧蛋白质向的关键调节者.
- 控制细胞两极分化的精确信号通路仍然不完全理解.
研究的目的:
- 调查Dishevelled (Dsh) 在调节Lgl局部化和细胞极性中的作用.
- 阐明Wnt信号通路在Lgl活动控制中的参与.
- 定义了细胞极性的Wnt介导调节的基础分子机制.
主要方法:
- 研究了Xenopus外皮和Drosophila毛囊表皮中的Lgl和Dishevelled (Dsh) 之间的关联.
- 利用Xenopus和Drosophila模型来研究Lgl和Dsh在确定顶点-基点极性的功能要求.
- 研究了Wnt受体Frizzled 8激活对Lgl局部化和活体活性的影响.
主要成果:
- 证明了一种脊椎动物Lgl同类物与Dishevelled (Dsh) 相联系.
- 显示Dsh调节Lgl在Xenopus外皮和Drosophila毛囊表皮中的局部化.
- 证实Lgl和Dsh都对Xenopus外皮细胞的正常基极性至关重要.
- 发现Frizzled 8的激活,但不是Frizzled 7,导致Lgl与皮质分离并失去其活性.
结论:
- 破碎 (Dsh) 在调节致命巨 (Lgl) 蛋白质的局部和活性方面发挥着至关重要的作用.
- 特别是通过Frizzled 8的Wnt信号,直接影响Lgl功能,提供Wnt通路和细胞极性之间的分子联系.
- 这些发现为了解Frizzled和Dishevelled在发育过程中如何协调细胞极性提供了一个新的分子框架.
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