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Updated: Jul 7, 2026

Combining Single-molecule Manipulation and Imaging for the Study of Protein-DNA Interactions
14:43

Combining Single-molecule Manipulation and Imaging for the Study of Protein-DNA Interactions

Published on: August 27, 2014

不平衡合成和组装混合无机蛋白纳米结构使用工程DNA结合蛋白质的合成和组装

Haixia Dai1, Woo-Seok Choe, Corrine K Thai

  • 1Departments of Chemical Engineering, Materials Science and Engineering, and Microbiology, University of Washington, Seattle, WA 98195-1750, USA.

Journal of the American Chemical Society
|November 3, 2005
PubMed
概括

研究人员设计了一种蛋白质,在非平衡条件下控制无机纳米粒子的形成. 这种蛋白质可以合成氧化铜 (Cu2O) 纳米粒子,模仿生物硬组织组装.

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Protein Complex Assembly02:41

Protein Complex Assembly

Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
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Protein Complex Assembly

Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
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Nucleic Acid Structure

The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms  a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA has a double-helix structure. The...