系统性炎症的转录和蛋白质图案在和离冠状动脉旁路移植中
V Tomic1, S Russwurm, E Möller
1Department of Anesthesiology and Intensive Care Medicine, Friedrich-Schiller-University, Jena, Germany.
Circulation
|November 9, 2005
概括
冠状动脉旁路移植 (CABG) 与心肺旁路 (CPB) 引发炎症. 离CABG可能会减少这种情况,但分子差异需要进一步研究以了解系统性影响.
科学领域:
- 心血管外科心血管外科
- 免疫学 免疫学 免疫学
- 分子生物学分子生物学
背景情况:
- 冠状动脉旁路移植 (CABG) 使用心肺旁路 (CPB) 提供受控的条件,但导致全身炎症.
- 离CABG (OPCAB) 避免了CPB,可能减少生理损伤,改善远程器官功能.
研究的目的:
- 研究OPCAB中保存的远程器官功能背后的分子机制.
- 为了比较基因表达和蛋白质基因反应的上与下CABG.
主要方法:
- 在白细胞中对基因表达模式的系统评估.
- 血蛋白质组分析以确定炎症媒介.
- 在上和下CABG患者反应的比较.
主要成果:
- CPB显著改变了45个转录,主要与炎症,细胞间通信和信号传递有关.
- 蛋白质组分析证实了已知的炎症媒介 (TNF-α,IL-6,IL-10) 并确定了其他 (IFN-γ,G-CSF).
- 外CABG显示一些细胞因子的释放延迟,循环白细胞在CPB接触后表现出"化"的表型.
结论:
- 结合基因阵列和蛋白质分析对于理解CPB的系统性影响至关重要.
- 循环白细胞过度表达CPB后的粘附和信号因子,可能导致肺陷和炎症.
- 这些发现突显了CPB诱导的分子变化,有助于致病率.
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