来自金属基板的表面启动聚合物的生物仿真
Xiaowu Fan1, Lijun Lin, Jeffrey L Dalsin
1Department of Biomedical Engineering, Robert R. McCormick School of Engineering and Applied Sciences, Northwestern University, 2145 Sheridan Road, Evanston, Illinois 60208, USA.
Journal of the American Chemical Society
|November 10, 2005
概括
研究人员开发了一种新的仿生启动器,用于在金属表面上制造抗聚合物涂层. 这一进步减少了细胞对和不钢的粘附,为先进的生物医学应用铺平了道路.
科学领域:
- 生物材料科学 生物材料科学
- 表面化学 表面化学
- 聚合物科学 聚合物科学
背景情况:
- 开发有效的防表面对于生物医学植入物和设备来说至关重要,以防止不必要的生物相互作用.
- 传统的表面修饰方法往往缺乏对金属基板的坚固粘附性,限制了涂层的耐用性.
- 贝类粘合蛋白为强大,多用途的表面 anchoring提供了一个自然模型.
研究的目的:
- 为了证明在金属表面上进行表面启动聚合 (SIP) 的开拓性启动器的首次使用.
- 为了创建强大的,抗的聚合物涂层,减少细胞粘附.
- 探索这种仿生方法在表面修饰方面的多功能性.
主要方法:
- 一个新的双功能启动器的合成,灵感来自于贝的粘合蛋白.
- 启动器在 (Ti) 和316L不钢 (SS) 基板上的表面吸附.
- 表面启动的原子转移激素聚合 (SI-ATRP) 使用固定启动器将甲基甲基酸盐巨型分子与脂乙烯糖醇 (OEG) 侧链接.
- 使用X射线光电子谱学 (XPS),表面FT-IR,圆测量和接触角度分析进行表征.
- 使用3T3-瑞士白化纤维细胞的细胞粘附研究.
- 使用SI-ATRP的微型图案和通过升起 (MAPL) 的分子组件图案.
主要成果:
- 催化剂启动剂强烈吸附于Ti和316LSS,使得聚合物移植成为可能.
- 通过SI-ATRP.成功地形成了高达100纳米厚的聚合物涂层.
- 与裸体金属相比,在聚合物涂层金属表面上观察到显著减少的纤维细胞粘附.
- 在Ti表面上成功地展示了抗涂料的微型涂料,创造出明显的细胞粘合和细胞抗性区域.
结论:
- 这项研究提出了一种新且多功能的生物模拟定策略,用于使用教化启动器进行表面修饰.
- 开发的防聚合物涂层通过最小化细胞粘附,显示出生物医学应用的巨大潜力.
- 该方法可适应各种无机表面,在薄膜表面修改中具有广泛的应用性.
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