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Updated: Jul 18, 2026

High-resolution Spatiotemporal Analysis of Receptor Dynamics by Single-molecule Fluorescence Microscopy
15:13

High-resolution Spatiotemporal Analysis of Receptor Dynamics by Single-molecule Fluorescence Microscopy

Published on: July 25, 2014

在表面上实时观察分子运动

Ellen H G Backus1, Andreas Eichler, Aart W Kleyn

  • 1Leiden Institute of Chemistry, Leiden University, Post Office Box 9502, 2300 RA Leiden, Netherlands.

Science (New York, N.Y.)
|November 12, 2005
PubMed
概括

超快速光谱学揭示了一氧化碳 (CO) 分子在上迅速移动. 这种快速的表面扩散是由旋转运动驱动的,而不仅仅是翻译,在500 femt秒的时间尺度上.

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Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...