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通过RNA聚合酶直接观察基对渐变的过程

Elio A Abbondanzieri1, William J Greenleaf, Joshua W Shaevitz

  • 1Department of Applied Physics, Stanford University, Stanford, California 94305, USA.

Nature
|November 15, 2005
PubMed
概括

在转录过程中,单个RNA聚合酶分子沿着DNA在一个基对的离散步骤中移动. 这种运动最好用布朗的杆模型来解释,而不是动力冲击模型.

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Real Time RT-PCR02:57

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Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...