Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

RNA polymerase inhibitors reveal active-site motions essential for the nucleotide addition cycle.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Capillary bundling of microtubules by condensates.

bioRxiv : the preprint server for biology·2026
Same author

ppGpp regulates transcription elongation via direct and indirect inputs to RNA polymerase pausing and nucleotide addition.

bioRxiv : the preprint server for biology·2026
Same author

RNAP-seq: <i>in vitro</i> genome-scale transcription reveals preferential RNA polymerase pausing on <i>Clostridioides difficile</i> antisense DNA.

bioRxiv : the preprint server for biology·2026
Same author

RNA polymerase inhibitors reveal active-site motions essential for the nucleotide-addition cycle.

bioRxiv : the preprint server for biology·2026
Same author

Cell-free genomics reveals fundamental regulatory principles of the Mycobacterium tuberculosis transcription cycle.

Molecular cell·2026

相关实验视频

Updated: May 8, 2026

Rab10 Phosphorylation Detection by LRRK2 Activity Using SDS-PAGE with a Phosphate-binding Tag
08:55

Rab10 Phosphorylation Detection by LRRK2 Activity Using SDS-PAGE with a Phosphate-binding Tag

Published on: December 14, 2017

14.9K

通过RNA聚合酶直接观察基对渐变的过程.

Elio A Abbondanzieri1, William J Greenleaf, Joshua W Shaevitz

  • 1Department of Applied Physics, Stanford University, Stanford, California 94305, USA.

Nature
|November 15, 2005
PubMed
概括

在转录过程中,单个RNA聚合酶分子沿着DNA在一个基对的离散步骤中移动. 这种运动最好用布朗的杆模型来解释,而不是动力冲击模型.

科学领域:

  • 分子生物学分子生物学
  • 生物物理学的生物物理.
  • 生物化学 生物化学

背景情况:

  • 转录是一种基本的生物过程,RNA聚合酶 (RNAP) 从DNA模板中合成RNA.
  • 了解RNAP在延长过程中的精确机械步骤对于破译基因调节至关重要.

研究的目的:

  • 开发一个超稳定的光学捕获系统,具有斯特罗姆级分辨率.
  • 为了监测大肠杆菌RNAP单个分子的转录延长.
  • 阐明RNAP在DNA上的转位的步骤大小和机制.

主要方法:

  • 开发一个超稳定的光学捕获系统,具有斯特罗姆级别的分辨率.
  • 通过大肠杆菌RNAP监测单分子转录.
  • 定量凝分析.
  • 在不同的核酸度下确定力-速度关系.

主要成果:

  • 单个RNAP分子以平均3.7±0.6 Å的离散步骤移动,这与B-DNA中每个基的增加一致.
  • RNAP沿着DNA向前推进,每增加一个核酸就有一对基因对.
  • 力量-速度数据支持一个布朗式子模型,其中有一个NTP的二次结合点.

更多相关视频

An Assay for Quantifying Protein-RNA Binding in Bacteria
07:02

An Assay for Quantifying Protein-RNA Binding in Bacteria

Published on: June 12, 2019

5.9K
Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using &#967;CRAC
09:15

Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC

Published on: May 9, 2020

6.1K

相关实验视频

Last Updated: May 8, 2026

Rab10 Phosphorylation Detection by LRRK2 Activity Using SDS-PAGE with a Phosphate-binding Tag
08:55

Rab10 Phosphorylation Detection by LRRK2 Activity Using SDS-PAGE with a Phosphate-binding Tag

Published on: December 14, 2017

14.9K
An Assay for Quantifying Protein-RNA Binding in Bacteria
07:02

An Assay for Quantifying Protein-RNA Binding in Bacteria

Published on: June 12, 2019

5.9K
Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using &#967;CRAC
09:15

Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC

Published on: May 9, 2020

6.1K

结论:

  • 在转录过程中RNAP转位发生在单个基对步骤中.
  • 布朗的拉切特模型为RNAP运动提供了比紧密合的动力冲击模型更好的解释.