基因组 H3 lysine 36 的转录组2 甲基化招募了一个抑制性的 Rpd3 复合体
Michael-Christopher Keogh1, Siavash K Kurdistani, Stephanie A Morris
1Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA.
Cell
|November 16, 2005
概括
酵母酵母Rpd3基因素脱乙酶在两个复合体中起作用. 具有Rco1和Eaf3子单元的Rpd3C(S) 复合体,通过Set2-介导的基因素甲基化被招募到脱乙酸转录DNA,调节基因表达.
科学领域:
- 分子生物学分子生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 酵母遗传学 酵母遗传学
背景情况:
- 酵母组织脱乙酶Rpd3 (含蛋白3的RPelA域) 在抑制转录启动的促销器中起着至关重要的作用.
- Rpd3在不同的蛋白质复合体中起作用,影响其调节作用.
研究的目的:
- 阐明酵母中两种不同的含Rpd3的复合物的组成和功能.
- 研究Rpd3C(S被招募到转录区域的机制及其在基因调节中的作用.
主要方法:
- 生物化学分析以确定Rpd3复杂成分.
- 基因研究来评估突变表型.
- 基因表达造型,以测量转录变化.
- 染色体免疫沉 (ChIP) 用于确定蛋白质-DNA相互作用.
- 历史素甲基化分析.
主要成果:
- Rpd3存在于两个复合体:Rpd3C(S) (小) 和Rpd3C(L) (大).
- Rpd3C(S) 含有独特的子单元Rco1和Eaf3,它的突变体表现出与Set2突变体相似的表型.
- Eaf3染色体调解了Rpd3C(S的招募到H3K36甲基化核体中,促进了转录区域的脱乙烯化.
- 删除Set2或Rpd3C(S) 基因可以绕过对正延长因子Bur1/Bur2.2的需求.
结论:
- 该Rpd3C(S) 复合物通过Set2-介导的基因素H3 lysine 36甲基化被招募到积极转录的基因中.
- 这种招募导致转录区域的脱乙基化,作为转录的负调节机制.
- 在酵母中,Set2-Rpd3C(S) 途径对于适当的转录延长控制至关重要.
相关概念视频
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02:38Heterochromatin
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02:32Histone Modification
The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone deacetylase,...
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone deacetylase,...