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相关概念视频

Histone Modification02:32

Histone Modification

The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone deacetylase,...
Spreading of Chromatin Modifications02:25

Spreading of Chromatin Modifications

The histone proteins in the nucleosomes are post-translationally modified (PTM) to increase or decrease access to DNA. The commonly observed PTMs are methylation, acetylation, phosphorylation, and ubiquitination of lysine amino acids in the histone H3 tail region. These histone modifications have specific meaning for the cell. Hence, they are called "histone code". The protein complex involved in histone modification is termed as "reader-writer" complex.
Writers
The writer is an enzyme that can...
RNA Polymerase II Accessory Proteins02:36

RNA Polymerase II Accessory Proteins

Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
Co-activators and Co-repressors02:04

Co-activators and Co-repressors

Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
Heterochromatin02:38

Heterochromatin

The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions that take up more dye are called heterochromatin. Heterochromatin is further classified into two forms – constitutive heterochromatin and facultative heterochromatin.
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at 9th...
Histone Modification02:32

Histone Modification

The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone deacetylase,...

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相关实验视频

Updated: May 11, 2026

Isolation and Cultivation of Neural Progenitors Followed by Chromatin-Immunoprecipitation of Histone 3 Lysine 79 Dimethylation Mark
10:09

Isolation and Cultivation of Neural Progenitors Followed by Chromatin-Immunoprecipitation of Histone 3 Lysine 79 Dimethylation Mark

Published on: January 26, 2018

基因组 H3 lysine 36 的转录组2 甲基化招募了一个抑制性的 Rpd3 复合体.

Michael-Christopher Keogh1, Siavash K Kurdistani, Stephanie A Morris

  • 1Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA.

Cell
|November 16, 2005
PubMed
概括

酵母酵母Rpd3基因素脱乙酶在两个复合体中起作用. 具有Rco1和Eaf3子单元的Rpd3C(S) 复合体,通过Set2-介导的基因素甲基化被招募到脱乙酸转录DNA,调节基因表达.

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10:44

In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing

Published on: May 5, 2023

相关实验视频

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Isolation and Cultivation of Neural Progenitors Followed by Chromatin-Immunoprecipitation of Histone 3 Lysine 79 Dimethylation Mark
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Chromatin Immunoprecipitation (ChIP) of Histone Modifications from Saccharomyces cerevisiae
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科学领域:

  • 分子生物学分子生物学
  • 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
  • 酵母遗传学 酵母遗传学

背景情况:

  • 酵母组织脱乙酶Rpd3 (含蛋白3的RPelA域) 在抑制转录启动的促销器中起着至关重要的作用.
  • Rpd3在不同的蛋白质复合体中起作用,影响其调节作用.

研究的目的:

  • 阐明酵母中两种不同的含Rpd3的复合物的组成和功能.
  • 研究Rpd3C(S被招募到转录区域的机制及其在基因调节中的作用.

主要方法:

  • 生物化学分析以确定Rpd3复杂成分.
  • 基因研究来评估突变表型.
  • 基因表达造型,以测量转录变化.
  • 染色体免疫沉 (ChIP) 用于确定蛋白质-DNA相互作用.
  • 历史素甲基化分析.

主要成果:

  • Rpd3存在于两个复合体:Rpd3C(S) (小) 和Rpd3C(L) (大).
  • Rpd3C(S) 含有独特的子单元Rco1和Eaf3,它的突变体表现出与Set2突变体相似的表型.
  • Eaf3染色体调解了Rpd3C(S的招募到H3K36甲基化核体中,促进了转录区域的脱乙烯化.
  • 删除Set2或Rpd3C(S) 基因可以绕过对正延长因子Bur1/Bur2.2的需求.

结论:

  • 该Rpd3C(S) 复合物通过Set2-介导的基因素H3 lysine 36甲基化被招募到积极转录的基因中.
  • 这种招募导致转录区域的脱乙基化,作为转录的负调节机制.
  • 在酵母中,Set2-Rpd3C(S) 途径对于适当的转录延长控制至关重要.