相关实验视频
Updated: Jul 7, 2026
14:57
Yeast As a Chassis for Developing Functional Assays to Study Human P53
Published on: August 4, 2019
卢西烯的总合成,一种细胞循环抑制剂,在p53-无活性的细胞中活跃
Robert S Coleman1, Matthew C Walczak, Erica L Campbell
1Department of Chemistry, The Ohio State University, 100 West 18th Avenue, Columbus, Ohio 43210-1185, USA. coleman@chemistry.ohio-state.edu
Journal of the American Chemical Society
|November 17, 2005
概括
研究人员用一种新型的异质二金属化1,3-二烯合成了抗瘤剂lucilactaene. 这种高效的八步总合成使用了顺序的Stille和Suzuki-Miyaura合器进行碎片组装.
科学领域:
- 有机合成 有机合成
- 药用化学 医学化学
- 有机金属化学 有机金属化学
背景情况:
- 露西烯是一种有前途的抗瘤剂.
- 复杂的聚天然产品的高效合成仍然是一个挑战.
研究的目的:
- 开发一个高效的lucilactaene的总合成.
- 在一个关键合策略中利用异质二金属化1,3-丁.
主要方法:
- 采用 hetero-bis-metalated 1,3-butadiene作为一个关键的合伙伴.
- 使用了顺序的Stille和苏苏基-Miyaura交叉合反应.
- 组装了lucilactaene的五合体侧链.
主要成果:
- 实现了lucilactaene的总合成.
- 合成仅在八个线性步骤中完成.
- 证明了/锡二烯在复杂分子合成中的实用性.
结论:
- 开发的合成路径非常高效.
- 异质二金属化二烯是构建聚烯系统的有价值的合成.
- 这种方法促进了对潜在的抗癌剂的获取.
相关概念视频
01:23Negative Regulator Molecules
Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
02:36DNA Damage can Stall the Cell Cycle
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
02:39Positive Regulator Molecules
Mitotic cell division results in daughter cells that exactly resemble the parent cell. However, errors in the DNA replication or distribution of genetic material may lead to genetic mutations that may be passed down to every new cell formed from the resulting abnormal cell. Propagation of such mutant cells is restricted through checkpoint mechanisms present at different stages of the cell cycle. These checkpoints involve regulator molecules that either promote or demote cell cycle events.
02:34Inhibition of Cdk Activity
The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
02:23Abnormal Proliferation
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...
02:36DNA Damage Can Stall the Cell Cycle
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...