通过TOR和Sch9来调节酵母复制寿命,以应对营养素
Matt Kaeberlein1, R Wilson Powers, Kristan K Steffen
1Departments of Genome Sciences and Medicine, University of Washington, Seattle, WA 98195, USA. kaeber@u.washington.edu
概括
卡路里限制可以延长酵母的寿命. 研究人员发现,删除TOR和Sch9通路中的基因也会增加寿命,这表明这些通路是营养感应和衰老的关键.
科学领域:
- * 衰老和长寿研究
- * 分子生物学和遗传学
- * 酵母作为一个模型生物体.
背景情况:
- * 卡路里限制 (CR) 是一种已知的干预措施,可以在各种生物体中延长寿命.
- * 了解CR作用背后的分子机制对于人类健康的研究至关重要.
- *酵母 (Saccharomyces cerevisiae) 作为一个强大的模型研究衰老,由于其遗传处理性和保存的衰老路径.
研究的目的:
- * 识别那些被删除后延长酵母复制寿命的基因.
- * 调查营养感应通路,特别是拉巴胺素的目标 (TOR) 和Sch9通路在酵母寿命中的作用.
- * 确定TOR和Sch9通路基因删除所赋予的寿命延长是否依赖于Sir2基因素脱乙酶.
主要方法:
- *对564个单基因删除酵母菌株进行大规模选.
- * 测量已识别的删除突变中的复制寿命.
- *基因分析涉及卡路里限制和TOR1,SCH9和SIR2基因的删除.
主要成果:
- *确定了10种基因删除,可以显著增加酵母复制寿命.
- *这些延长寿命的删除中有6个涉及TOR和Sch9营养反应通路的组件编码基因.
- * 卡路里限制没有进一步延长tor1Δ或sch9Δ突变的寿命,而TOR1或SCH9删除独立于Sir2.9延长了寿命.
结论:
- *TOR和Sch9激酶通路是酵母中长寿的关键调节者.
- *这些通路是营养摄入影响寿命的主要机制.
- *删除TOR1或SCH9可以延长寿命,突出显示它们在老化过程中的作用,独立于Sir2.
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