TCP1复合体是氨酸生物生成中的分子伴侣
M B Yaffe1, G W Farr, D Miklos
1Department of Pharmacology, Case Western Reserve School of Medicine, Cleveland, Ohio 44106.
Nature
|July 16, 1992
概括
T复合型多-1 (TCP1) 作为细胞质中新合成的蛋白蛋白的伴侣. 这种伴侣功能对于tubulin来说至关重要,以实现微管组件的正确形状.
科学领域:
- 细胞质蛋白折叠 细胞质蛋白折叠
- 分子的伴侣是分子的伴侣.
- 微管子生物发生.
背景情况:
- T复杂多-1 (TCP1) 涉及到真核生物中细胞质蛋白质的折叠.
- TCP1的特定分子标仍然未被确定.
- 结核素对于微管形成至关重要,但其子单元的折叠情况尚不清楚.
研究的目的:
- 为了研究TCP1在新翻译的α-和β-tubulin子单元的生物发生中的作用.
- 为了识别细胞质中TCP1的分子点.
- 为了阐明管在早期折叠过程中所经历的形状变化.
主要方法:
- 在子网状细胞溶解酸中研究了蛋白生物发生.
- 使用蛋白酶敏感性测试来评估蛋白质构成.
- 使用大小排除色谱 (900K复合体) 分析了蛋白质复合体.
- 通过抗体交叉反应识别了该复合体的主要蛋白质成分.
主要成果:
- 在一种对蛋白酶敏感的900K复合体中发现了新合成的氨酸子单元.
- 添加Mg-ATP会释放出突素子单元,形成一种具有组装能力的,抗蛋白酶的形式.
- 900K复合体主要含有由抗TCP1抗体识别的58K蛋白质.
- 确定TCP1是复杂结合新翻译的管蛋白的主要组成部分.
结论:
- TCP1的功能是作为一个细胞分裂的陪伴者,专门用于管生物发生.
- TCP1有助于获得管组装的适当形状.
- 这项研究确定了氨酸是TCP1的分子标,澄清了它在细胞骨蛋白质成熟中的作用.
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