T-复杂聚-1 是真核细胞细胞质中异构粒子的一个子单元
V A Lewis1, G M Hynes, D Zheng
1Institute of Cancer Research, Chester Beatty Laboratories, London, UK.
Nature
|July 16, 1992
概括
T复合多-1 (TCP1) 是一种细胞质蛋白质,在一个独特的异质寡合分子粒子中发现. 这个粒子这个粒子.
科学领域:
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
- 生物化学 生物化学
背景情况:
- T复合体多-1 (TCP1) 是由小鼠t复合体编码的,在大多数细胞中表达,特别是在精子生成过程中高调.
- 哺乳动物TCP1序列表现出彼此高度相同 (>96%) 和酵母和Drosophila Orthologues显著相同 (>60%),表明功能保留.
- TCP1在酵母中是必不可少的,并假设它是哺乳动物细胞溶液相当于groEL,一个分子陪伴者.
研究的目的:
- 描述鼠类和人类TCP1的原生结构和组成.
- 研究TCP1作为细胞蛋白折叠机制的潜在作用.
主要方法:
- 负染色电子显微镜被用来确定TCP1粒子的结构特征.
- 生物化学分析被用来识别本地TCP1复合体内的相关蛋白质.
主要成果:
- 鼠和人类的TCP1存在于细胞质中作为一个大型异质寡合粒子 (800K-950K).
- 这颗粒子与四到六个未识别的蛋白质和两个Hsp70热冲击蛋白质有关.
- 电子显微镜揭示了一个独特的结构,两个堆叠的环,直径12-16纳米.
结论:
- 尽管TCP1与Chaperonin 60蛋白质有相似之处,但在生物化学和结构上是不同的.
- TCP1可能是参与蛋白质折叠的新型真核细胞分子家族的一部分.
- 异构组合可能在调节TCP1功能的过程中发挥作用.
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