通过体自我组装构建的非扭曲β-sheet纤维的层状形态
Matthew S Lamm1, Karthikan Rajagopal, Joel P Schneider
1Department of Materials Science and Engineering, the Delaware Biotechnology Institute, Newark, Delaware 19716, USA.
Journal of the American Chemical Society
|November 25, 2005
概括
一种新型的合成可以自组装成高度有序的2Dβ片纤维. 这种受控的组合提供了与粉样纤维相似的稳定性和特性.
科学领域:
- 生物材料科学 生物材料科学
- 超分子化学 超分子化学
- 体自组装器 自组装器
背景情况:
- 粉样纤维素与各种疾病有关.
- 了解自我组装机制对于设计新型生物材料至关重要.
- De novo的设计允许对结构和功能进行精确的控制.
研究的目的:
- 设计和描述一种可以自组装成有序,非扭曲的β片纤维的合成.
- 研究设计的纤维的形态,稳定性和组装动力学.
- 确定决定观察到的纤维细胞形态的关键结构特征.
主要方法:
- 一个新的的设计和合成.
- 传输电子显微镜 (TEM) 用于形态分析.
- 动态光散射 (DLS) 和循环二元化 (CD) 谱学用于结构特征.
- 动力学研究改变pH值和温度以控制组件.
主要成果:
- 一个合成被设计成可以自组装成 2.5 nm 宽的统一的细丝.
- 这些细丝横向结合成平面,层状结构,其宽度超过50纳米,长度超过微米.
- 纤维的高度是精确控制在7nm,相当于一个单个单体在扩展的β-链形状.
- 由此产生的二维结构在广泛的pH值和温度范围内表现出高稳定性.
- 组装速率和层叠度可通过pH值和温度进行调节.
- 体内的二林序列被确定为促进非扭曲,层状形态的关键.
结论:
- 新设计的可以自组装成高度有序的2D纤维状结构,具有类似粉样蛋白的特征.
- 对形态和稳定性的精确控制为生物材料和纳米技术中的应用提供了可能性.
- 双连接器在指导非扭曲的,层状组装路径方面发挥着关键作用.
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