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Updated: Jul 6, 2026

Anaerobic Protein Purification and Kinetic Analysis via Oxygen Electrode for Studying DesB Dioxygenase Activity and Inhibition
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Anaerobic Protein Purification and Kinetic Analysis via Oxygen Electrode for Studying DesB Dioxygenase Activity and Inhibition

Published on: October 3, 2018

对catchol 1,2-dioxygenase的无氧酶基质复合物的光谱研究

Geoff P Horsman1, Andrew Jirasek, Frédéric H Vaillancourt

  • 1Department of Biochemistry, The University of British Columbia, Vancouver, Canada.

Journal of the American Chemical Society
|December 1, 2005
PubMed
概括

这项研究揭示了catechol以dianion的形式与铁结合在内二氧化酶中,与外酶不同. 这一发现得到了光谱学和DFT的支持,澄清了酶机制和基质结合.

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