相关实验视频
Updated: May 11, 2026
12:19
Analysis of Cell Cycle Position in Mammalian Cells
Published on: January 21, 2012
该APC/C和CBP/p300合作调节转录和细胞循环进展
Andrew S Turnell1, Grant S Stewart, Roger J A Grand
1Cancer Research UK Institute for Cancer Studies, The Medical School, The University of Birmingham, Edgbaston, Birmingham B15 2TT, UK. A.S.Turnell@bham.ac.uk
Nature
|December 2, 2005
概括
亚纳酶促进复合体/循环体 (APC/C) 与CBP/p300联合激活剂相互作用,影响细胞周期进展和转录. 这种相互作用对APC/C功能至关重要,并影响细胞转化.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 亚纳酶促进复合体/循环体 (APC/C) 是一个关键的E3泛基因酶,调节细胞循环的进展通过线粒分裂和G1.
- 虽然它在细胞循环控制中的作用已经确立,但其他APC/C功能仍然不太了解.
研究的目的:
- 为了研究超出细胞周期调节的APC/C组件的新功能.
- 阐明APC/C与协同激活剂CBP/p300之间的相互作用及其功能后果.
主要方法:
- 蛋白质与蛋白质相互作用试验用于研究APC5/APC7和CBP/p300结合.
- 测试测量CBP/p300乙转移酶活性和转录活性.
- 通过RNA介导的干扰来评估CBP在APC/C功能中的作用.
- 分析E1A介导的细胞转化.
主要成果:
- APC/C组件APC5和APC7通过保留的域直接与协同激活剂CBP和p300相互作用.
- 这种相互作用增强了CBP/p300乙转移酶的活性,并增强了转录.
- APC5和APC7以CBP/p300依赖的方式抑制E1A介导的转化.
- CBP对于APC/C活性至关重要,因为其耗尽会降低E3结合酶活性和线性进展.
结论:
- APC/C组件APC5和APC7与CBP/p300形成功能复合体,影响转录和细胞转化.
- 这些发现揭示了APC/C在生长调节中的新角色,并表明APC/C组件在转化过程中可能是目标.
- CBP是APC/C泛基因酶活性和细胞周期进展的关键调节者.
相关概念视频
01:23Negative Regulator Molecules
Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
02:11The Cell Cycle Control System
The cell cycle is an organized set of events that leads the cell to divide into two daughter cells, each containing chromosomes identical to the parent cell. It is the cell cycle that leads to the formation of an entire organism from a single-cell zygote. Besides, cell division also functions in the renewal or repair of tissues in adult multicellular eukaryotes. For example, in the bone marrow, the stem cells divide to form new blood cells. Although essential for several functions, cell...
00:50Anaphase Promoting Complex
The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
02:11The Cell Cycle Control System
The cell cycle is an organized set of events that leads the cell to divide into two daughter cells, each containing chromosomes identical to the parent cell. It is the cell cycle that leads to the formation of an entire organism from a single-cell zygote. Besides, cell division also functions in the renewal or repair of tissues in adult multicellular eukaryotes. For example, in the bone marrow, the stem cells divide to form new blood cells. Although essential for several functions, cell...
00:50Anaphase Promoting Complex
The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
01:28The Cell Cycle Control System
The cell cycle regulation directs how a cell proceeds from one phase to the next and begins mitosis. The cell cycle control system includes intracellular regulatory molecules and external triggers. They provide "stop" or "advance" signals and operate at specific cell cycle stages termed checkpoints to ensure that a particular process is completed before the cell advances to the next phase.
Cyclins and cyclin-dependent kinases (Cdks) are the primary cell cycle regulators and function at the cell...
Cyclins and cyclin-dependent kinases (Cdks) are the primary cell cycle regulators and function at the cell...