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相关概念视频

Cytoskeletal Coordination in Cell Migration01:32

Cytoskeletal Coordination in Cell Migration

A migrating cell changes its shape during the cyclic events of attachment and detachment from the substratum and repositions the cell organelles correspondingly. These complex events are orchestrated by the dynamic cytoskeletal network comprising actin filaments, intermediate filaments, and microtubules. Cytoskeletal crosstalk — the direct and indirect communication between the different components — is crucial for this coordination. Direct communication involves various linker proteins that...
Structure of Cadherins01:25

Structure of Cadherins

The cadherins were one of the first cell adhesion molecules discovered; the term “cadherins”   is based on their calcium-dependent adhering properties. The first cadherins discovered on the epithelial, neuronal, and placental cells were named E-cadherin, P-cadherin, and N-cadherin, respectively. These classical cadherins share sequence and structural similarities. Other cadherins, including those involved in cell signaling, are grouped into non-classical cadherins. This diversity of cadherins...
Catenins01:23

Catenins

Catenins are characterized by multiple binding domains and dynamic structures that allow them to function as linker proteins in cell junction complexes. All catenins, except α-catenin, contain a characteristic protein sequence called the armadillo repeat and are therefore also called armadillo proteins.
Catenins in Cell Junctions
Catenins bind to cell adhesion molecules such as cadherins and link them to different cytoskeletal proteins depending on the type of cell junction. At the adherens...
Adherens Junctions01:24

Adherens Junctions

Strong contact points between adjacent cells anchor them to each other, forming tissues. Such anchoring junctions are of two types –  adherens junctions and desmosomes. Adherens junctions are abundant in tissues such as  epithelium and endothelium, forming a continuous zone of adhesion called the adhesion belt. In other tissues, such as  heart muscle, they appear as clusters, linking the cells to produce coordinated heart muscle contraction.
Adherens Junctions are Dynamic
The endothelial cells...
Cadherins in Tissue Organization01:19

Cadherins in Tissue Organization

The cadherins are a superfamily of cell adhesion molecules comprising over 180 variants, with specific tissues expressing a particular combination of cadherin types. Cadherins generally exhibit homophilic binding; i.e., cadherins on one cell bind to cadherins of the same or closely related type on another cell. Thus, cells of the same type have a specific affinity to bind to each other and sort themselves into clusters to form tissues.
Cell Sorting During Development
Cell sorting plays an...
Tension Response at Adherens Junctions01:26

Tension Response at Adherens Junctions

The adherens junctions that anchor cells together are multi-protein complexes that dynamically adapt to mechanical stimuli such as tensile forces and shear stress. Mechanosensory proteins in these junctions can sense such mechanical stimuli and undergo a shift in their conformation, resulting in an altered function — a process called mechanotransduction.
α-Catenin as a Mechanosensory Protein
The α-catenin of adherens junctions is an allosteric protein with three VH (vinculin homology) domains...

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相关实验视频

Updated: May 11, 2026

Reconstitution Of β-catenin Degradation In Xenopus Egg Extract
09:41

Reconstitution Of β-catenin Degradation In Xenopus Egg Extract

Published on: June 18, 2014

阿尔法-catenin 是一个分子开关,它结合E-cadherin-beta-catenin,并调节actin-filament组件.

Frauke Drees1, Sabine Pokutta, Soichiro Yamada

  • 1Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, CA 94305, USA.

Cell
|December 6, 2005
PubMed
概括
此摘要是机器生成的。

对于细胞粘附至关重要的α-catenin存在两种形式:单体和二元. 这些形式具有独特的结合性质,影响着actin细胞骨组织和细胞结节稳定性.

更多相关视频

Bead Aggregation Assays for the Characterization of Putative Cell Adhesion Molecules
08:15

Bead Aggregation Assays for the Characterization of Putative Cell Adhesion Molecules

Published on: October 17, 2014

Measuring Protein Binding to F-actin by Co-sedimentation
06:17

Measuring Protein Binding to F-actin by Co-sedimentation

Published on: May 18, 2017

相关实验视频

Last Updated: May 11, 2026

Reconstitution Of β-catenin Degradation In Xenopus Egg Extract
09:41

Reconstitution Of β-catenin Degradation In Xenopus Egg Extract

Published on: June 18, 2014

Bead Aggregation Assays for the Characterization of Putative Cell Adhesion Molecules
08:15

Bead Aggregation Assays for the Characterization of Putative Cell Adhesion Molecules

Published on: October 17, 2014

Measuring Protein Binding to F-actin by Co-sedimentation
06:17

Measuring Protein Binding to F-actin by Co-sedimentation

Published on: May 18, 2017

科学领域:

  • 细胞生物学 细胞生物学
  • 分子生物学分子生物学
  • 生物化学 生物化学

背景情况:

  • 皮质细胞-细胞结点通过粘附蛋白和actin细胞骨架保持组织完整性.
  • 以前认为α-catenin可以将E-cadherin-beta-catenin复合体与行为丝连接起来.

研究的目的:

  • 为了研究α-catenin在细胞-细胞粘附中的结合特性和调节作用.
  • 阐明α-catenin影响actin细胞骨架的机制.

主要方法:

  • 生物化学测试以确定α-catenin的结合亲和力.
  • 对α-catenin分子构造的分析.
  • 对α-catenin对Arp2/3复合体活动的影响的研究.

主要成果:

  • 阿尔法-素存在于具有明显结合偏好的单体和同体体形式.
  • 单体α-catenin与E-cadherin-beta-catenin结合,而二元性α-catenin则与actin纤维结合. 单体α-catenin与E-cadherin-beta-catenin结合,而二元性α-catenin与actin纤维结合.
  • 阿尔法-氨酸通过抑制Arp2/3复杂介导的氨酸组合来调节氨酸聚合.

结论:

  • 阿尔法-素作为一种具有依赖于形状的结合的全蛋白质起作用.
  • α-catenin在细胞-细胞粘附部位的actin组织的局部调节中发挥着新的作用.