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相关概念视频

Eukaryotic RNA Polymerases00:58

Eukaryotic RNA Polymerases

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RNA Polymerase (RNAP) is conserved in all animals, with bacterial, archaeal, and eukaryotic RNAPs sharing significant sequence, structural, and functional similarities. Among the three eukaryotic RNAPs, RNA Polymerase II is most similar to bacterial RNAP in terms of both structural organization and folding topologies of the enzyme subunits. However, these similarities are not reflected in their mechanism of action.
All three eukaryotic RNAPs require specific transcription factors, of which the...
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RNA-seq03:21

RNA-seq

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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相关实验视频

Updated: May 3, 2026

Capture and Identification of RNA-binding Proteins by Using Click Chemistry-assisted RNA-interactome Capture CARIC Strategy
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Capture and Identification of RNA-binding Proteins by Using Click Chemistry-assisted RNA-interactome Capture CARIC Strategy

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追踪RNA聚合酶,一个步骤在一个时间.

Dmitry G Vassylyev1, Irina Artsimovitch

  • 1Department of Biochemistry and Molecular Genetics, University of Alabama, Birmingham, Alabama 35294, USA.

Cell
|December 20, 2005
PubMed
概括

研究人员发现,细菌RNA聚合酶以单基对步骤移动. 这种酶的功能就像布朗的子,在转录过程中由核酸三酸盐结合推进.

科学领域:

  • 分子生物学分子生物学
  • 生物物理学的生物物理.

背景情况:

  • 对转录的结构分析已经实现了高分辨率.
  • 在转录中操纵单个分子的技术是有限的.

研究的目的:

  • 开发和应用单分子操纵技术来研究转录.
  • 阐明转录过程中细菌RNA聚合酶的阶段性机制.

主要方法:

  • 利用单分子力光谱测量单个细菌RNA聚合酶分子的运动.
  • 分析了DNA沿线聚合酶转位的步骤大小和动态.

主要成果:

  • 证明细菌RNA聚合酶以离散的,单基对步骤转移.
  • 观察到,传入的核酸三酸盐的结合驱动了聚合酶向前运动.

结论:

  • 细菌RNA聚合酶的功能就像一个布朗的子.
  • 核酸的结合能量被转化为机械工作,以驱动转录进展.

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