Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

12.1K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
12.1K
Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

2.6K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.6K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Optimization of the Quantum-Si Platinum Single-Molecule Protein Sequencing Platform Toward Improved Complex-Matrix Protein Identification.

ACS omega·2026
Same author

Demonstration of <i>in vivo</i> efficacy, cryo-EM-epitope identification, and breadth of two anti-alphavirus bispecific single domain antibodies.

Journal of virology·2025
Same author

Engineered phages for selective adsorption of rare earth elements.

Scientific reports·2025
Same author

A single domain antibody-based Luminex assay for the detection of SARS-CoV-2 in clinical samples.

Frontiers in immunology·2024
Same author

Probing Protein Corona Formation around Gold Nanoparticles: Effects of Surface Coating.

ACS nano·2024
Same author

Investigating the dissipation of heat and quantum information from DNA-scaffolded chromophore networks.

The Journal of chemical physics·2024

相关实验视频

Updated: Jan 9, 2026

Using In Vitro Fluorescence Resonance Energy Transfer to Study the Dynamics Of Protein Complexes at a Millisecond Time Scale
10:50

Using In Vitro Fluorescence Resonance Energy Transfer to Study the Dynamics Of Protein Complexes at a Millisecond Time Scale

Published on: March 14, 2019

8.6K

基于量子点的多重光共振能量转移.

Aaron R Clapp1, Igor L Medintz, H Tetsuo Uyeda

  • 1U.S. Naval Research Laboratory, Optical Sciences Division, Code 5611, Washington, DC 20375, USA.

Journal of the American Chemical Society
|December 22, 2005
PubMed
概括

我们展示了发光量子点 (QD) 如何将能量传递给蛋白质受体,以便进行多重检测. 使用多个QD捐赠器与一个接受器简化了数据分析,用于同时进行能量转移测量.

科学领域:

  • 生物物理学的生物物理.
  • 材料科学 材料科学 材料科学
  • 分析化学 分析化学

背景情况:

  • 非辐射能量转移 (NRET) 对生物过程和传感至关重要.
  • 量子点 (QD) 为生物成像和测试提供可调节的光学特性.
  • 多重检测需要高效和可区分的能量传输系统.

研究的目的:

  • 为了在QD和蛋白质受体之间以多重格式展示NRET.
  • 为了比较QD蛋白NRET的两个配置:单个QD多个受体和多个QD单个受体.
  • 评估每个配置数据分析的简单性.

主要方法:

  • 将发光QDs与染料标记的蛋白质受体相结合.
  • 使用稳定状态和时间分辨率的光谱学.
  • 调查两个NRET配置:单个QD与多个接受器和多个QD与一个接受器.

主要成果:

  • 在两个探索的配置中成功测量了同时的NRET.
  • 具有多个QD捐赠者和一个接受者类型的配置提供了更简单的数据分析.
  • 时间解析测量证实了参与NRET群体的选择性QD寿命缩短.

更多相关视频

Compact Quantum Dots for Single-molecule Imaging
17:14

Compact Quantum Dots for Single-molecule Imaging

Published on: October 9, 2012

18.6K
Combining QD-FRET and Microfluidics to Monitor DNA Nanocomplex Self-Assembly in Real-Time
14:36

Combining QD-FRET and Microfluidics to Monitor DNA Nanocomplex Self-Assembly in Real-Time

Published on: August 26, 2009

11.6K

相关实验视频

Last Updated: Jan 9, 2026

Using In Vitro Fluorescence Resonance Energy Transfer to Study the Dynamics Of Protein Complexes at a Millisecond Time Scale
10:50

Using In Vitro Fluorescence Resonance Energy Transfer to Study the Dynamics Of Protein Complexes at a Millisecond Time Scale

Published on: March 14, 2019

8.6K
Compact Quantum Dots for Single-molecule Imaging
17:14

Compact Quantum Dots for Single-molecule Imaging

Published on: October 9, 2012

18.6K
Combining QD-FRET and Microfluidics to Monitor DNA Nanocomplex Self-Assembly in Real-Time
14:36

Combining QD-FRET and Microfluidics to Monitor DNA Nanocomplex Self-Assembly in Real-Time

Published on: August 26, 2009

11.6K

结论:

  • 对于多重测定,QD蛋白NRET是可行的.
  • 选择NRET配置会影响数据分析的复杂性.
  • 时间解析的光提供了对NRET事件的强有力的验证.