Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Organization of Genes02:07

Organization of Genes

Overview
What is Gene Expression?01:42

What is Gene Expression?

Overview
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...
Chromatin Position Affects Gene Expression02:35

Chromatin Position Affects Gene Expression

Chromatin is the massive complex of DNA and proteins packaged inside the nucleus. The complexity of chromatin folding and how it is packaged inside the nucleus greatly influences  access to genetic information. Generally, the nucleus' periphery is considered transcriptionally repressive, while the cell's interior is considered a transcriptionally active area. 
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the timing and level of...
Exon Recombination02:32

Exon Recombination

The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes. 
Exon shuffling follows “splice frame rules.” Each exon has three reading...
What is Gene Expression?01:36

What is Gene Expression?

A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is comprised  of nucleotides and proteins are comprised of amino acids, a mediator is required to convert the information encoded in DNA into proteins. This mediator is the messenger RNA (mRNA). mRNA copies the blueprint from DNA by a process called transcription. In eukaryotes, transcription occurs in the nucleus by complementary base-pairing with the DNA template. The mRNA is then processed and...
Structure of a Gene01:30

Structure of a Gene

A gene is the fundamental unit of heredity. Every individual has two copies of each gene, one inherited from each parent. Although most people contain the same genes, there is a small fraction that is slightly different amongst people. A gene with a small difference in its sequence of DNA bases forms different alleles, contributing to different phenotypes.
However, only 1% of the DNA is composed of genes that encode proteins; the rest, 99% is non-coding DNA. This non-coding DNA performs...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Nocturnal Features and Daytime Characteristics in Narcolepsy: Reliability and Diagnostic Relevance for NT1 vs NT2.

Sleep·2026
Same author

A novel, wearable, in-ear EEG technology to assess sleep and daytime sleepiness.

Bioelectronic medicine·2026
Same author

Methods for Wearable-Derived Pulse Rate Measures and Their Application to Modeling the Relationship between Pulse Rate and Motor Activity in Narcolepsy Type 1.

Digital biomarkers·2026
Same author

BudFinder: A Masked Auto-Encoder vision transformer framework for yeast budding detection and lifespan quantification.

PLoS computational biology·2026
Same author

Functional Regression Methods for Estimating 24-Hour Ambulatory Blood Pressure and Heart Rate Parameters in Narcolepsy Type 1.

Digital biomarkers·2026
Same author

Bacteria as living biosensors for DNA.

Nature reviews bioengineering·2026

相关实验视频

Updated: Jun 30, 2026

Processing of Primary Brain Tumor Tissue for Stem Cell Assays and Flow Sorting
08:14

Processing of Primary Brain Tumor Tissue for Stem Cell Assays and Flow Sorting

Published on: September 25, 2012

在真核生物基因表达的外部变异性的起源.

Dmitri Volfson1, Jennifer Marciniak, William J Blake

  • 1Department of Bioengineering, University of California San Diego, La Jolla, California 92093, USA.

Nature
|December 24, 2005
PubMed
概括

细胞多样性源于基因表达的变异性. 这项研究确定了外在变异的两个主要来源,包括人群动态和调节网络,这对于理解细胞差异至关重要.

科学领域:

  • 分子生物学分子生物学
  • 系统生物学 系统生物学
  • 遗传学 是一个遗传学.

背景情况:

  • 克隆细胞群体内的可变基因表达影响着诸如进化和疾病之类的关键生物过程.
  • 外在因素对表达变异性有显著的贡献,影响多个基因,但它们的起源仍未得到充分研究.

研究的目的:

  • 为了确定外部基因表达变化的生物学起源.
  • 研究基因调控网络如何促进细胞多样性.

主要方法:

  • 使用了Saccharomyces cerevisiae的计算建模和光数据.
  • 分析了来自多个促进基因插入的数据,以量化表达变异性.

主要成果:

  • 确定了两个外部变异的主要来源:基因表达与人口动态的合以及一个共同的上游转录因子.
  • 证明了监管网络可以放大上游监管器的噪音,从而导致目标基因中的外部噪音.
  • 建立了表达因人口动态变化的下限.

结论:

  • 外在基因表达的变化源于人口动态和监管网络结构.
  • 了解基因调节网络和人口异质性之间的相互作用是解释细胞多样性的关键.

更多相关视频

Single-cell Gene Expression Profiling Using FACS and qPCR with Internal Standards
10:50

Single-cell Gene Expression Profiling Using FACS and qPCR with Internal Standards

Published on: February 25, 2017

Combined Nucleotide and Protein Extractions in Caenorhabditis elegans
10:37

Combined Nucleotide and Protein Extractions in Caenorhabditis elegans

Published on: March 17, 2019

相关实验视频

Last Updated: Jun 30, 2026

Processing of Primary Brain Tumor Tissue for Stem Cell Assays and Flow Sorting
08:14

Processing of Primary Brain Tumor Tissue for Stem Cell Assays and Flow Sorting

Published on: September 25, 2012

Single-cell Gene Expression Profiling Using FACS and qPCR with Internal Standards
10:50

Single-cell Gene Expression Profiling Using FACS and qPCR with Internal Standards

Published on: February 25, 2017

Combined Nucleotide and Protein Extractions in Caenorhabditis elegans
10:37

Combined Nucleotide and Protein Extractions in Caenorhabditis elegans

Published on: March 17, 2019